Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1973 Jan;51(1):146–149. doi: 10.1104/pp.51.1.146

Hormonal Control of Isoperoxidases in Lentil Embryonic Axis 1

Thomas Gaspar 1,2, Anwar A Khan 1,2, Danielle Fries 1,2,2
PMCID: PMC367372  PMID: 16658276

Abstract

Detachment of the cotyledons from the lentil (Lens culinaris Med.) embryonic axis causes in the latter an increase in total peroxidase activity which is shown to be due to enhancement of specific cathodic isoperoxidases. Kinetin treatment of attached or detached axes promotes activity of essentially the same cathodic isoperoxidases. In addition kinetin enhances the activity of two anodic peroxidases and represses specifically that of a cathodic one. Abscisic acid inhibits the production of all isoenzymes in the presence or absence of kinetin. Cytokinin and abscisic acid actions are discussed in relation to the nature of a wounding hormone and the role of natural inhibitors in cotyledons during germination. Indoleacetic acid stimulates the activity of certain isoenzymes which are also stimulated by kinetin, whereas in others the effects of the two hormones are different. Specific inverse effects of indoleacetic acid and kinetin are demonstrated on the two most cathodic isoperoxidases. Indoleacetic acid-kinetin interactions on the cathodic isoperoxidases have been found in the literature and are discussed as a possible mechanism for explaining interactions of the two regulators on growth and other physiological processes.

Full text

PDF
146

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenfeld A., Gazit S. Interaction of kinetin and abscisic Acid in the growth of soybean callus. Plant Physiol. 1970 Apr;45(4):535–536. doi: 10.1104/pp.45.4.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galston A. W., Davies P. J. Hormonal regulation in higher plants. Science. 1969 Mar 21;163(3873):1288–1297. doi: 10.1126/science.163.3873.1288. [DOI] [PubMed] [Google Scholar]
  3. Gaspar T., Khan A. A., Bouchet M. Isoperoxidases in the lentil embryonic axis grown attached and detached from the cotyledons. Arch Int Physiol Biochim. 1972 Jan;80(1):187–188. [PubMed] [Google Scholar]
  4. Khan A. A. Cytokinins: permissive role in seed germination. Science. 1971 Mar 5;171(3974):853–859. doi: 10.1126/science.171.3974.853. [DOI] [PubMed] [Google Scholar]
  5. Khann A. A., Dubucq M., Gaspar T., Roe C. H. Peroxidase synthesis in embryonic axis of lentil grown attached and detached from the cotyledons. Arch Int Physiol Biochim. 1972 Jan;80(1):195–196. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lee T. T. Cytokinin-controlled Indoleacetic Acid Oxidase Isoenzymes in Tobacco Callus Cultures. Plant Physiol. 1971 Feb;47(2):181–185. doi: 10.1104/pp.47.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee T. T. Promotion of indoleacetic Acid oxidase isoenzymes in tobacco callus cultures by indoleacetic Acid. Plant Physiol. 1971 Jul;48(1):56–59. doi: 10.1104/pp.48.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ockerse R., Siegel B. Z., Galston A. W. Hormone-induced repression of a peroxidase isozyme in plant tissue. Science. 1966 Jan 28;151(3709):452–453. doi: 10.1126/science.151.3709.452. [DOI] [PubMed] [Google Scholar]
  10. Scopes R. K. Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species. Biochem J. 1968 Mar;107(2):139–150. doi: 10.1042/bj1070139. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES