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. 1974 Sep;54(3):320–323. doi: 10.1104/pp.54.3.320

Temperature-induced Phase Changes in the Membranes of Glyoxysomes, Mitochondria, and Proplastids from Germinating Castor Bean Endosperm 1

Neal L Wade a,2, R William Breidenbach a, James M Lyons a, Alec D Keith b
PMCID: PMC367404  PMID: 16658881

Abstract

Sucrose-gradient purified mitochondria, glyoxysomes, and proplastids from germinating castor bean (Ricinus communis L.) endosperm were examined by electron-spin resonance spectroscopy. A temperature-induced phase change was demonstrated in all of these organelles, their derivative membranes, and in micelles formed from the membrane phospholipids. The apparent transition temperature of the membrane lipids varied slightly between the samples, but in all cases, fell within the temperature range around 10 C where physiological and biochemical changes in the response to temperature for most chilling-sensitive plants occur.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Breidenbach R. W., Beevers H. Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm. Biochem Biophys Res Commun. 1967 May 25;27(4):462–469. doi: 10.1016/s0006-291x(67)80007-x. [DOI] [PubMed] [Google Scholar]
  3. Breidenbach R. W., Kahn A., Beevers H. Characterization of glyoxysomes from castor bean endosperm. Plant Physiol. 1968 May;43(5):705–713. doi: 10.1104/pp.43.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Breidenbach R. W., Wade N. L., Lyons J. M. Effect of chilling temperatures on the activities of glyoxysomal and mitochondrial enzymes from castor bean seedlings. Plant Physiol. 1974 Sep;54(3):324–327. doi: 10.1104/pp.54.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Douglass S. A., Criddle R. S., Breidenbach R. W. Characterization of deoxyribonucleic Acid species from castor bean endosperm: inability to detect a unique deoxyribonucleic Acid species associated with glyoxysomes. Plant Physiol. 1973 May;51(5):902–906. doi: 10.1104/pp.51.5.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keith A. D., Waggoner A. S., Griffith O. H. Spin-labeled mitochondrial lipids in Neurospora crassa. Proc Natl Acad Sci U S A. 1968 Nov;61(3):819–826. doi: 10.1073/pnas.61.3.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  9. Raison J. K., Lyons J. M., Mehlhorn R. J., Keith A. D. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J Biol Chem. 1971 Jun 25;246(12):4036–4040. [PubMed] [Google Scholar]
  10. Raison J. K., Lyons J. M. The influence of mitochondrial concentration and storage on the respiratory control of isolated plant mitochondria. Plant Physiol. 1970 Apr;45(4):382–385. doi: 10.1104/pp.45.4.382. [DOI] [PMC free article] [PubMed] [Google Scholar]

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