Table 2. Primers used in qPCR.
| Gene | Forward sequence (5′→3′) | Reverse sequence (5′→3′) | Accession (GenBank) | Size (bp) | E (%) |
|---|---|---|---|---|---|
|
myog |
GTCACAGGAACAGAGGACAAAG |
TGGTCACTGTCTTCCTTTTGC |
EU934044 |
118 |
92 |
|
dnmt1 |
GATCCCAGTGAGGAGTACGG |
AAGAAGGTCCTCATAAGTAGCGTC |
KC129104 |
117 |
93 |
|
dnmt3a |
AACTGCTGTAGGTGTTTCTGTGTG |
CGCCGCAGTAACCCGTAG |
KC129105 |
134 |
90 |
|
dnmt3b |
ATCAAGCGATGTGGCGAGC |
CGATGCGGTGAAAGTCAGTCC |
KC129106 |
91 |
97 |
|
eef1a1 |
ATTGGCGGCATTGGAACA |
CATCTCCACAGACTTGACCTC |
AB326302 |
117 |
91 |
|
rps4 |
CTGCTGGATTCATGGATGTG |
GGCAGTGATGCGGTGGAC |
AB291557 |
101 |
90 |
| ubq | TCTGCGTGGTGGTCTCATC | TGACCACACTTCTTCTTGCG | AB291588 | 135 | 92 |
For each gene, its GenBank accession number, amplicon size (bp) and amplification efficiency (E) are indicated. The annealing temperature of all primer pairs is 60°C.