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. 1974 Sep;54(3):408–411. doi: 10.1104/pp.54.3.408

Ultrastructural Evidence for Calcium Phosphate Deposition by Isolated Corn Shoot Mitochondria 1

John H Peverly a,2, Raymond J Miller a,3, Carl Malone a, David E Koeppe a,4
PMCID: PMC367422  PMID: 16658899

Abstract

This study demonstrates that isolated corn (Zea mays L. Wf9 xM14) shoot mitochondria will accumulate calcium phosphate crystals in vitro that are comparable to those observed in both in vivo and in vitro animal mitochondria. The intensity of deposition, as observed by electron microscopy, increased with incubation time, substrate concentration, and concentration of calcium and inorganic phosphate. Calcium phosphate deposits were initially observed as amorphous, nearly spherical areas that lacked sharp or clear edges, but which were crystalline in nature under heavier loading conditions and longer incubation times.

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Selected References

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  1. GREENAWALT J. W., ROSSI C. S., LEHNINGER A. L. EFFECT OF ACTIVE ACCUMULATION OF CALCIUM AND PHOSPHATE IONS ON THE STRUCTURE OF RAT LIVER MITOCHONDRIA. J Cell Biol. 1964 Oct;23:21–38. doi: 10.1083/jcb.23.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Greenawalt J. W., Carafoli E. Electron microscope studies on the active accumulation of Sr++ by rat-liver mitochondria. J Cell Biol. 1966 Apr;29(1):37–61. doi: 10.1083/jcb.29.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hanson J. B., Miller R. J. Evidence for active phosphate transport in maize mitochondria. Proc Natl Acad Sci U S A. 1967 Aug;58(2):727–734. doi: 10.1073/pnas.58.2.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Leggett J. E., Galloway R. A., Gauch H. G. Calcium Activation of Orthophosphate Absorption by Barley Roots. Plant Physiol. 1965 Sep;40(5):897–902. doi: 10.1104/pp.40.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lehninger A. L., Carafoli E., Rossi C. S. Energy-linked ion movements in mitochondrial systems. Adv Enzymol Relat Areas Mol Biol. 1967;29:259–320. doi: 10.1002/9780470122747.ch6. [DOI] [PubMed] [Google Scholar]
  7. Miller R. J., Dumford S. W., Koeppe D. E., Hanson J. B. Divalent cation stimulation of substrate oxidation by corn mitochondria. Plant Physiol. 1970 Jun;45(6):649–653. doi: 10.1104/pp.45.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller R. J., Koeppe D. E. The effect of calcium and inhibitors on corn mitochondrial respiration. Plant Physiol. 1971 Jun;47(6):832–835. doi: 10.1104/pp.47.6.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ramirez-Mitchell R., Johnson H. M., Wilson R. H. Strontium deposits in isolated plant mitochondria. Exp Cell Res. 1973 Feb;76(2):449–451. doi: 10.1016/0014-4827(73)90400-x. [DOI] [PubMed] [Google Scholar]

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