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. 1974 Oct;54(4):520–526. doi: 10.1104/pp.54.4.520

Uptake of Bicarbonate Ion in Darkness by Isolated Chloroplast Envelope Membranes and Intact Chloroplasts of Spinach

Raymond P Poincelot a
PMCID: PMC367445  PMID: 16658920

Abstract

Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates the usefulness of the envelope membranes for the study of chloroplast permeability. Maximal rates for dark HCO3- uptake by isolated envelope membranes and intact chloroplasts were more than sufficient to account for the maximal rates of photosynthetic CO2 fixation observed with intact chloroplasts. The active species involved in the uptake process was found to be HCO3- and not CO2. The significance of HCO3- uptake and its relationship to carbonic anhydrase and ribulose diphosphate carboxylase is discussed. Conditions for maximal HCO3- uptake in darkness by intact chloroplasts were found to be similar to those required for maximal photosynthetic CO2 fixation, suggesting that HCO3- uptake by the envelope membrane may regulate photosynthetic CO2 fixation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUINSMA J. A comment on the spectrophotometric determination of chlorophyll. Biochim Biophys Acta. 1961 Sep 30;52:576–578. doi: 10.1016/0006-3002(61)90418-8. [DOI] [PubMed] [Google Scholar]
  2. Cooper T. G., Filmer D. The active species of "CO2" utilized by ribulose diphosphate carboxylase. J Biol Chem. 1969 Feb 10;244(3):1081–1083. [PubMed] [Google Scholar]
  3. Cooper T. G., Tchen T. T., Wood H. G., Benedict C. R. The carboxylation of phosphoenolpyruvate and pyruvate. I. The active species of "CO2" utilized by phosphoenolpyruvate carboxykinase, carboxytransphosphorylase, and pyruvate carboxylase. J Biol Chem. 1968 Jul 25;243(14):3857–3863. [PubMed] [Google Scholar]
  4. Douce R., Holtz R. B., Benson A. A. Isolation and properties of the envelope of spinach chloroplasts. J Biol Chem. 1973 Oct 25;248(20):7215–7222. [PubMed] [Google Scholar]
  5. Heldt H. W., Rapley L. Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett. 1970 Oct 5;10(3):143–148. doi: 10.1016/0014-5793(70)80438-0. [DOI] [PubMed] [Google Scholar]
  6. Heldt H. W., Sauer F. The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim Biophys Acta. 1971 Apr 6;234(1):83–91. doi: 10.1016/0005-2728(71)90133-2. [DOI] [PubMed] [Google Scholar]
  7. Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095–1101. doi: 10.1073/pnas.56.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Nelson N., Nelson H., Racker E. Partial resolution of the enzymes catalyzing photophosphorylation. XI. Magnesium-adenosine triphosphatase properties of heat-activated coupling factor I from chloroplasts. J Biol Chem. 1972 Oct 25;247(20):6506–6510. [PubMed] [Google Scholar]
  10. Poincelot R. P. Differences in lipid composition between intact and membrane-stripped spinach chlorplasts. Biochim Biophys Acta. 1971 Jun 8;239(1):57–60. doi: 10.1016/0005-2760(71)90192-5. [DOI] [PubMed] [Google Scholar]
  11. Walker D. A. Correlation between Photosynthetic Activity and Membrane Integrity in Isolated Pea Chloroplasts. Plant Physiol. 1965 Nov;40(6):1157–1161. doi: 10.1104/pp.40.6.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Werdan K., Heldt H. W. Accumulation of bicarbonate in intact chloroplasts following a pH gradient. Biochim Biophys Acta. 1972 Dec 14;283(3):430–441. doi: 10.1016/0005-2728(72)90260-5. [DOI] [PubMed] [Google Scholar]

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