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. 1974 Oct;54(4):569–574. doi: 10.1104/pp.54.4.569

Physiological Studies with Isolated Leaf Cells

Early Products of Photosynthesis and Their Metabolic Interconversions

G Kulandaivelu a, A Gnanam a
PMCID: PMC367455  PMID: 16658930

Abstract

A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Crotalaria Laburnifolia L., and Thunbergia grandiflora Roxb.

By pulse-chase time course experiments, the photosynthetically fixed primary carbon compounds of bean leaf cells were not converted into the ethanol-insoluble fraction. About 25% of total 14C-photoassimilates were found to leak out into the incubation medium. In contrast, Euglena and Chlorella cells incorporated their primary photosynthetic products into cellular macromolecules and the amount of “leak” was very little. 14C-Leucine supplied to the bean cells was absorbed readily and incorporated into the trichloroacetic acid insoluble fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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