Abstract
Nitrogenase activity, determined by the acetylene reduction assay, in soybean cell suspensions infected with Rhizobium, was stimulated significantly by the addition of succinic acid or glutamine. Acetylene-dependent ethylene values as high as 4.6 μmoles of ethylene/gram dry weight·hour were observed.
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Selected References
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- Child J. J., Larue T. A. A simple technique for the establishment of nitrogenase in soybean callus culture. Plant Physiol. 1974 Jan;53(1):88–90. doi: 10.1104/pp.53.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy R. W., Holsten R. D., Jackson E. K., Burns R. C. The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43(8):1185–1207. doi: 10.1104/pp.43.8.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holsten R. D., Burns R. C., Hardy R. W., Hebert R. R. Establishment of symbiosis between Rhizobium and plant cells in vitro. Nature. 1971 Jul 16;232(5307):173–176. doi: 10.1038/232173a0. [DOI] [PubMed] [Google Scholar]
- Koch B., Evans H. J., Russell S. Properties of the nitrogenase system in cell-free extracts of bacteroids from soybean root nodules. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1343–1350. doi: 10.1073/pnas.58.4.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips D. A. Factors affecting the reduction of acetylene by Rhizobium-soybean cell associations in vitro. Plant Physiol. 1974 Jan;53(1):67–72. doi: 10.1104/pp.53.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]