Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1986 Jan;6(1):315–317. doi: 10.1128/mcb.6.1.315

Liver-specific expression of a Qa-encoded class I gene is associated with DNA hypomethylation.

C G Miyada, R B Wallace
PMCID: PMC367512  PMID: 3023831

Abstract

DNA methylation of two murine major histocompatibility complex (H-2) class I genes was examined in hybridizations to MspI and HpaII chromosomal DNA restriction digests. Q10, which exhibits liver-specific expression, and H-2Kb, a transplantation antigen gene, were examined in liver, spleen, thymus, and cell-line DNAs. Unmethylated Q10 gene sequences were detected only in the liver, whereas the H-2Kb gene was unmethylated in all tissues examined.

Full text

PDF
315

Images in this article

316Image
on p.316
317Image
on p.317

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barra Y., Tanaka K., Isselbacher K. J., Khoury G., Jay G. Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen. Mol Cell Biol. 1985 Jun;5(6):1295–1300. doi: 10.1128/mcb.5.6.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Feldmann G. Morphologic aspects of hepatic synthesis and secretion of plasma proteins. Prog Liver Dis. 1979;6:23–41. [PubMed] [Google Scholar]
  3. Hood L., Steinmetz M., Malissen B. Genes of the major histocompatibility complex of the mouse. Annu Rev Immunol. 1983;1:529–568. doi: 10.1146/annurev.iy.01.040183.002525. [DOI] [PubMed] [Google Scholar]
  4. Hsiao W. L., Gattoni-Celli S., Kirschmeier P., Weinstein I. B. Effects of 5-azacytidine on methylation and expression of specific DNA sequences in C3H 10T1/2 cells. Mol Cell Biol. 1984 Apr;4(4):634–641. doi: 10.1128/mcb.4.4.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klein J., Juretic A., Baxevanis C. N., Nagy Z. A. The traditional and a new version of the mouse H-2 complex. Nature. 1981 Jun 11;291(5815):455–460. doi: 10.1038/291455a0. [DOI] [PubMed] [Google Scholar]
  6. Kress M., Cosman D., Khoury G., Jay G. Secretion of a transplantation-related antigen. Cell. 1983 Aug;34(1):189–196. doi: 10.1016/0092-8674(83)90149-6. [DOI] [PubMed] [Google Scholar]
  7. Mellor A. L., Weiss E. H., Kress M., Jay G., Flavell R. A. A nonpolymorphic class I gene in the murine major histocompatibility complex. Cell. 1984 Jan;36(1):139–144. doi: 10.1016/0092-8674(84)90082-5. [DOI] [PubMed] [Google Scholar]
  8. Miyada C. G., Klofelt C., Reyes A. A., McLaughlin-Taylor E., Wallace R. B. Evidence that polymorphism in the murine major histocompatibility complex may be generated by the assortment of subgene sequences. Proc Natl Acad Sci U S A. 1985 May;82(9):2890–2894. doi: 10.1073/pnas.82.9.2890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orlofsky A., Chasin L. A. A domain of methylation change at the albumin locus in rat hepatoma cell variants. Mol Cell Biol. 1985 Jan;5(1):214–225. doi: 10.1128/mcb.5.1.214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Razin A., Riggs A. D. DNA methylation and gene function. Science. 1980 Nov 7;210(4470):604–610. doi: 10.1126/science.6254144. [DOI] [PubMed] [Google Scholar]
  11. Riggs A. D., Jones P. A. 5-methylcytosine, gene regulation, and cancer. Adv Cancer Res. 1983;40:1–30. doi: 10.1016/s0065-230x(08)60678-8. [DOI] [PubMed] [Google Scholar]
  12. Schulze D. H., Pease L. R., Obata Y., Nathenson S. G., Reyes A. A., Ikuta S., Wallace R. B. Identification of the cloned gene for the murine transplantation antigen H-2Kb by hybridization with synthetic oligonucleotides. Mol Cell Biol. 1983 Apr;3(4):750–755. doi: 10.1128/mcb.3.4.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Singer J., Roberts-Ems J., Riggs A. D. Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II. Science. 1979 Mar 9;203(4384):1019–1021. doi: 10.1126/science.424726. [DOI] [PubMed] [Google Scholar]
  14. Steinmetz M., Winoto A., Minard K., Hood L. Clusters of genes encoding mouse transplantation antigens. Cell. 1982 Mar;28(3):489–498. doi: 10.1016/0092-8674(82)90203-3. [DOI] [PubMed] [Google Scholar]
  15. Tanaka K., Appella E., Jay G. Developmental activation of the H-2K gene is correlated with an increase in DNA methylation. Cell. 1983 Dec;35(2 Pt 1):457–465. doi: 10.1016/0092-8674(83)90179-4. [DOI] [PubMed] [Google Scholar]
  16. Tsao S. G., Brunk C. F., Pearlman R. E. Hybridization of nucleic acids directly in agarose gels. Anal Biochem. 1983 Jun;131(2):365–372. doi: 10.1016/0003-2697(83)90185-9. [DOI] [PubMed] [Google Scholar]
  17. Waalwijk C., Flavell R. A. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. Nucleic Acids Res. 1978 Sep;5(9):3231–3236. doi: 10.1093/nar/5.9.3231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiss E. H., Golden L., Fahrner K., Mellor A. L., Devlin J. J., Bullman H., Tiddens H., Bud H., Flavell R. A. Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse. Nature. 1984 Aug 23;310(5979):650–655. doi: 10.1038/310650a0. [DOI] [PubMed] [Google Scholar]
  19. Weiss E., Golden L., Zakut R., Mellor A., Fahrner K., Kvist S., Flavell R. A. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens. EMBO J. 1983;2(3):453–462. doi: 10.1002/j.1460-2075.1983.tb01444.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES