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. 1986 Feb;6(2):488–493. doi: 10.1128/mcb.6.2.488

Extramitochondrial citrate synthase activity in bakers' yeast.

T M Rickey, A S Lewin
PMCID: PMC367537  PMID: 3023850

Abstract

We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.

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Selected References

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