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. 1986 Feb;6(2):586–592. doi: 10.1128/mcb.6.2.586

Cellular response to DNA damage is enhanced by the pR plasmid in mouse cells and in Escherichia coli.

L Marcucci, F Gigliani, P A Battaglia, R Bosi, E Sporeno, R Elli
PMCID: PMC367549  PMID: 3023858

Abstract

The pR plasmid, which enhances the survival of Escherichia coli C600 exposed to UV light by induction of the SOS regulatory mechanism, showed the same effect when it transformed mouse LTA cells (tk-, aprt-). With Tn5 insertion mutagenesis which inactivates UV functions in the pR plasmid, we recognized two different regions of the plasmid, uvp1 and uvp2. These pR UVR- mutants exhibited the same effect in LTA transformed cells, demonstrating that resistance to UV light, carried by the pR plasmid, was really due to the expression of these two regions, which were also in the mouse cells. Statistical analysis showed that the expression of the uvp1 and uvp2 regions significantly increased (P less than 0.01) the survival upon exposure to UV light in mouse cells and bacteria. These results might suggest the presence of an inducible repair response to DNA damage in mouse LTA cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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