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. 1986 Feb;6(2):703–706. doi: 10.1128/mcb.6.2.703

Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

F Toneguzzo, A C Hayday, A Keating
PMCID: PMC367561  PMID: 3466022

Abstract

The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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