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. 1986 Apr;6(4):1276–1282. doi: 10.1128/mcb.6.4.1276

Diverse long terminal repeats are associated with murine retroviruslike (VL30) elements.

A Itin, E Keshet
PMCID: PMC367640  PMID: 3023879

Abstract

The VL30 family is a retroviruslike gene family with no apparent nucleic acid homology to any known retrovirus. Over 100 copies of VL30 DNA elements are dispersed throughout the mouse genome. Sequence analysis of the VL30 long terminal repeat (LTR) units showed that, whereas the LTR units of a given VL30 DNA element were almost identical, the LTR units associated with distinct members of the family were very different from one another. Comparison of the LTR sequences possessed by two particular VL30 DNA elements revealed a pattern of extensively homologous DNA segments adjacent to only distantly related DNA sequences. With the aid of sub-LTR probes, it was shown that a certain LTR is composed of both U5 sequences that are abundantly present in all species of the genus Mus and a U3 region detected only in Mus musculus. In addition, we isolated a VL30 DNA element in which the LTR units were replaced by the LTR units of an apparently novel retroviruslike family. These findings suggest that recombinations have played a role in generating the diverse population of VL30-associated LTRs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Besmer P., Olshevsky U., Baltimore D., Dolberg D., Fan H. Virus-like 30S RNA in mouse cells. J Virol. 1979 Mar;29(3):1168–1176. doi: 10.1128/jvi.29.3.1168-1176.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boeke J. D., Garfinkel D. J., Styles C. A., Fink G. R. Ty elements transpose through an RNA intermediate. Cell. 1985 Mar;40(3):491–500. doi: 10.1016/0092-8674(85)90197-7. [DOI] [PubMed] [Google Scholar]
  3. Brûlet P., Kaghad M., Xu Y. S., Croissant O., Jacob F. Early differential tissue expression of transposon-like repetitive DNA sequences of the mouse. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5641–5645. doi: 10.1073/pnas.80.18.5641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coffin J. M., Champion M., Chabot F. Nucleotide sequence relationships between the genomes of an endogenous and an exogenous avian tumor virus. J Virol. 1978 Dec;28(3):972–991. doi: 10.1128/jvi.28.3.972-991.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coffin J. M. Structure, replication, and recombination of retrovirus genomes: some unifying hypotheses. J Gen Virol. 1979 Jan;42(1):1–26. doi: 10.1099/0022-1317-42-1-1. [DOI] [PubMed] [Google Scholar]
  6. Courtney M. G., Elder P. K., Steffen D. L., Getz M. J. Evidence for an early evolutionary origin and locus polymorphism of mouse VL30 DNA sequences. J Virol. 1982 Aug;43(2):511–518. doi: 10.1128/jvi.43.2.511-518.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ellis R. W., DeFeo D., Maryak J. M., Young H. A., Shih T. Y., Chang E. H., Lowy D. R., Scolnick E. M. Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus. J Virol. 1980 Nov;36(2):408–420. doi: 10.1128/jvi.36.2.408-420.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hodgson C. P., Elder P. K., Ono T., Foster D. N., Getz M. J. Structure and expression of mouse VL30 genes. Mol Cell Biol. 1983 Dec;3(12):2221–2231. doi: 10.1128/mcb.3.12.2221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Howk R. S., Troxler D. H., Lowy D., Duesberg P. H., Scolnick E. M. Identification of a 30S RNA with properties of a defective type C virus in murine cells. J Virol. 1978 Jan;25(1):115–123. doi: 10.1128/jvi.25.1.115-123.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hunter E. The mechanism for genetic recombination in the avian retroviruses. Curr Top Microbiol Immunol. 1978;79:295–309. doi: 10.1007/978-3-642-66853-1_7. [DOI] [PubMed] [Google Scholar]
  11. Itin A., Keshet E. Apparent recombinants between virus-liKE (VL30) and murine leukemia virus-related sequences in mouse DNA. J Virol. 1983 Jul;47(1):178–184. doi: 10.1128/jvi.47.1.178-184.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Itin A., Keshet E. Nucleotide sequence analysis of the long terminal repeat of murine virus-like DNA (VL30) and its adjacent sequences: resemblance to retrovirus proviruses. J Virol. 1983 Sep;47(3):656–659. doi: 10.1128/jvi.47.3.656-659.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Itin A., Rotman G., Keshet E. Conservation patterns of mouse "virus-like" (VL30) DNA sequences. Virology. 1983 Jun;127(2):374–384. doi: 10.1016/0042-6822(83)90151-4. [DOI] [PubMed] [Google Scholar]
  14. Keshet E., Itin A. Patterns of genomic distribution and sequence heterogeneity of a murine "retrovirus-like" multigene family. J Virol. 1982 Jul;43(1):50–58. doi: 10.1128/jvi.43.1.50-58.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuff E. L., Feenstra A., Lueders K., Rechavi G., Givol D., Canaani E. Homology between an endogenous viral LTR and sequences inserted in an activated cellular oncogene. Nature. 1983 Apr 7;302(5908):547–548. doi: 10.1038/302547a0. [DOI] [PubMed] [Google Scholar]
  16. Larson R., Messing J. Apple II software for M13 shotgun DNA sequencing. Nucleic Acids Res. 1982 Jan 11;10(1):39–49. doi: 10.1093/nar/10.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lueders K. K., Kuff E. L. Sequences associated with intracisternal A particles are reiterated in the mouse genome. Cell. 1977 Dec;12(4):963–972. doi: 10.1016/0092-8674(77)90161-1. [DOI] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  19. Norton J. D., Connor J., Avery R. J. Unusual long terminal repeat sequence of a retrovirus transmissible mouse (VL 30) genetic element: identification of functional domains. Nucleic Acids Res. 1984 Apr 25;12(8):3445–3460. doi: 10.1093/nar/12.8.3445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scolnick E. M., Vass W. C., Howk R. S., Duesberg P. H. Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus. J Virol. 1979 Mar;29(3):964–972. doi: 10.1128/jvi.29.3.964-972.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sherwin S. A., Rapp U. R., Benveniste R. E., Sen A., Todaro G. J. Rescue of endogenous 30S retroviral sequences from mouse cells by baboon type C virus. J Virol. 1978 May;26(2):257–264. doi: 10.1128/jvi.26.2.257-264.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wirth T., Glöggler K., Baumruker T., Schmidt M., Horak I. Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3327–3330. doi: 10.1073/pnas.80.11.3327. [DOI] [PMC free article] [PubMed] [Google Scholar]

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