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. 1986 Apr;6(4):1352–1356. doi: 10.1128/mcb.6.4.1352

The Saccharomyces cerevisiae chromosome III left telomere has a type X, but not a type Y', ARS region.

L L Button, C R Astell
PMCID: PMC367652  PMID: 3537702

Abstract

A yeast Saccharomyces cerevisiae telomeric region was isolated by chromosome walking from HML alpha, the most distal known gene on the chromosome III left (IIIL) end. The terminal heterodisperse 3.3-kilobase (kb) SalI fragment on chromosome IIIL, 8.6 kb distal to HML alpha, was cloned in a circular vector to generate a telomeric probe. Southern hybridization and DNA sequencing analyses indicated that 0.6 kb (+/- 200 base pairs) of 5'-C1-3A-3' simple tandem repeat sequence, adjacent to a 1.2-kb type X ARS region, constitutes the telomere on the chromosome IIIL end, and no type Y' ARS region homologies exist between HML alpha and the IIIL terminus.

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Selected References

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