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MiR-181b overexpression regulates IGF-1R signaling. (A) Levels of p-Akt, Akt, p-ERK1/2, ERK, HIF-1α, and GAPDH protein were detected by Western blot analysis. (B) VEGF mRNA levels were determined by qRT-PCR and normalized to those of GAPDH. (*) Significant difference when compared with control (P < 0.05).
