FIGURE 5.

S4 Mutations affect the structures of 5WJ complexes. (A) Secondary structure representations of the 16S 5WJ indicating the relative SHAPE reactivity (τ) of complexes with S4 mutants relative to WT S4 complexes, as indicated in each panel. Color scheme as in Figure 4E. Residues that do not show a significant difference in reactivity in mutant vs. WT complexes are light gray. (B) Relative reactivity (τ) depicted on the mature 16S structure in E. coli ribosomes (PDB 2AVY). Top panels show the “530 loop” of H18 loop; bottom panels show the H18 pseudoknot.