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. 1986 Jun;6(6):2098–2105. doi: 10.1128/mcb.6.6.2098

Specific protein binding to the simian virus 40 enhancer in vitro.

A G Wildeman, M Zenke, C Schatz, M Wintzerith, T Grundström, H Matthes, K Takahashi, P Chambon
PMCID: PMC367750  PMID: 3023918

Abstract

HeLa cell nuclear extracts and wild-type or mutated simian virus 40 enhancer DNA were used in DNase I footprinting experiments to study the interaction of putative trans-acting factors with the multiple enhancer motifs. We show that these nuclear extracts contain proteins that bind to these motifs. Because point mutations which are detrimental to the activity of a particular enhancer motif in vivo specifically prevent protection of that motif against DNase I digestion in vivo, we suggest that the bound proteins correspond to trans-acting factors involved in enhancement of transcription. Using mutants in which the two domains A and B of the simian virus 40 enhancer are either separated by insertion of DNA fragments or inverted with respect to their natural orientation, we also demonstrate that the trans-acting factors bind independently to the two domains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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