. Author manuscript; available in PMC: 2013 Jun 10.

Published in final edited form as: Biochemistry. 2011 Jun 3;50(25):5757–5766. doi: 10.1021/bi2001856

Table 1.

Summary of measured rates, activation entropy and enthalpy of the reactions as well as the calculated activation free energy in the absence of E3 and in the presence of wild-type and different E3 mutants with insertions.

	k (M⁻¹ s⁻¹)^*	ΔH^≠ (kcal/mol)	TΔS^≠ (kcal/mol)^*	ΔG^≠ (kcal)^*
E3	(1.1 +/− 0.4) × 10⁵	24.0 +/− 2.4	12.9 +/− 2.2	11.0 +/− 3.3
E3_GG insertion	(1.2 +/− 0.5) × 10⁴	14.7 +/− 2.7	2.2 +/− 2.4	12.4 +/− 3.6
E3_linker insertion	(1.8 +/− 0.6) × 10³	12.0 +/− 1.1	−1.4 +/− 0.9	13.6 +/− 1.4
No E3 activity	(1.7 +/− 0.6) × 10²	10.7 +/− 0.3	−4.3 +/− 0.3	15.0 +/− 0.4

Values correspond to those measured at 37°C.