Abstract
Human gamma interferon genomic DNA was introduced into NIH 3T3 fibroblasts by calcium phosphate precipitation and was not expressed in these cells at the cytoplasmic mRNA or protein level. Treatment of the transfected cells with cycloheximide (1 microgram/ml) induced the accumulation of cytoplasmic gamma interferon mRNA and biologically active human gamma interferon. Analysis of the nuclear enriched RNA from untreated cells indicated that human gamma interferon mRNA was present, suggesting that cycloheximide may act by inhibiting a specific nuclease or may enhance the processing or transport of the RNA from the nucleus to the cytoplasm.
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Selected References
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- Armstrong J. A. Cytopathic effect inhibition assay for interferon: microculture plate assay. Methods Enzymol. 1981;78(Pt A):381–387. doi: 10.1016/0076-6879(81)78145-x. [DOI] [PubMed] [Google Scholar]
- Cavalieri R. L., Havell E. A., Vilcek J., Pestka S. Induction and decay of human fibroblast interferon mRNA. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4415–4419. doi: 10.1073/pnas.74.10.4415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cross F. R., Darnell J. E., Jr Cycloheximide stimulates early adenovirus transcription if early gene expression is allowed before treatment. J Virol. 1983 Feb;45(2):683–692. doi: 10.1128/jvi.45.2.683-692.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Efrat S., Kaempfer R. Control of biologically active interleukin 2 messenger RNA formation in induced human lymphocytes. Proc Natl Acad Sci U S A. 1984 May;81(9):2601–2605. doi: 10.1073/pnas.81.9.2601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farrar W. L., Johnson H. M., Farrar J. J. Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2. J Immunol. 1981 Mar;126(3):1120–1125. [PubMed] [Google Scholar]
- Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., Howard B. H. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777–6781. doi: 10.1073/pnas.79.22.6777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
- Gray P. W., Goeddel D. V. Structure of the human immune interferon gene. Nature. 1982 Aug 26;298(5877):859–863. doi: 10.1038/298859a0. [DOI] [PubMed] [Google Scholar]
- Gurney T., Jr Characterization of mouse 45S ribosomal RNA subspecies suggests that the first processing cleavage occurs 600 +/- 100 nucleotides from the 5' end and the second 500 +/- 100 nucleotides from the 3' end of a 13.9 kb precursor. Nucleic Acids Res. 1985 Jul 11;13(13):4905–4919. doi: 10.1093/nar/13.13.4905. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamilton R. T., Nilsen-Hamilton M., Adams G. Superinduction by cycloheximide of mitogen-induced secreted proteins produced by Balb/c 3T3 cells. J Cell Physiol. 1985 May;123(2):201–208. doi: 10.1002/jcp.1041230208. [DOI] [PubMed] [Google Scholar]
- Ishihara T., Kudo A., Watanabe T. Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment. J Exp Med. 1984 Dec 1;160(6):1937–1942. doi: 10.1084/jem.160.6.1937. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luthman H., Magnusson G. High efficiency polyoma DNA transfection of chloroquine treated cells. Nucleic Acids Res. 1983 Mar 11;11(5):1295–1308. doi: 10.1093/nar/11.5.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Makino R., Hayashi K., Sugimura T. C-myc transcript is induced in rat liver at a very early stage of regeneration or by cycloheximide treatment. Nature. 1984 Aug 23;310(5979):697–698. doi: 10.1038/310697a0. [DOI] [PubMed] [Google Scholar]
- Maroteaux L., Chen L., Mitrani-Rosenbaum S., Howley P. M., Revel M. Cycloheximide induces expression of the human interferon beta 1 gene in mouse cells transformed by bovine papillomavirus-interferon beta 1 recombinants. J Virol. 1983 Jul;47(1):89–95. doi: 10.1128/jvi.47.1.89-95.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nir U., Cohen B., Chen L., Revel M. A human IFN-beta 1 gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA. Nucleic Acids Res. 1984 Sep 25;12(18):6979–6993. doi: 10.1093/nar/12.18.6979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ringold G. M., Dieckmann B., Vannice J. L., Trahey M., McCormick F. Inhibition of protein synthesis stimulates the transcription of human beta-interferon genes in Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3964–3968. doi: 10.1073/pnas.81.13.3964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schloss J. A., Silflow C. D., Rosenbaum J. L. mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii. Mol Cell Biol. 1984 Mar;4(3):424–434. doi: 10.1128/mcb.4.3.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sehgal P. B., Lyles D. S., Tamm I. Superinduction of human fibroblast interferon production: further evidence for increased stability of interferon mRNA. Virology. 1978 Aug;89(1):186–198. doi: 10.1016/0042-6822(78)90051-x. [DOI] [PubMed] [Google Scholar]
- Sorrentino V., Battistini A., Curatola A. M., Di Francesco P., Rossi G. B. Induction and/or selective retention of proteins in mammalian cells exposed to cycloheximide. J Cell Physiol. 1985 Nov;125(2):313–318. doi: 10.1002/jcp.1041250221. [DOI] [PubMed] [Google Scholar]
- Southern P. J., Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982;1(4):327–341. [PubMed] [Google Scholar]
- Stimac E., Groppi V. E., Coffino P. Increased histone mRNA levels during inhibition of protein synthesis. Biochem Biophys Res Commun. 1983 Jul 18;114(1):131–137. doi: 10.1016/0006-291x(83)91604-2. [DOI] [PubMed] [Google Scholar]
- Tan Y. H., Armstrong J. A., Ke Y. H., Ho M. Regulation of cellular interferon production: enhancement by antimetabolites. Proc Natl Acad Sci U S A. 1970 Sep;67(1):464–471. doi: 10.1073/pnas.67.1.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tan Y. H., Berthold W. A mechanism for the induction and regulation of human fibroblastoid interferon genetic expression. J Gen Virol. 1977 Mar;34(3):401–411. doi: 10.1099/0022-1317-34-3-401. [DOI] [PubMed] [Google Scholar]
- Thomas P. S. Hybridization of denatured RNA transferred or dotted nitrocellulose paper. Methods Enzymol. 1983;100:255–266. doi: 10.1016/0076-6879(83)00060-9. [DOI] [PubMed] [Google Scholar]
- Trinchieri G., Matsumoto-Kobayashi M., Clark S. C., Seehra J., London L., Perussia B. Response of resting human peripheral blood natural killer cells to interleukin 2. J Exp Med. 1984 Oct 1;160(4):1147–1169. doi: 10.1084/jem.160.4.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vilcek J., Ng M. H. Post-transcriptional control of interferon synthesis. J Virol. 1971 May;7(5):588–594. doi: 10.1128/jvi.7.5.588-594.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]