FIG. 4.

Cell proliferation and cell death in IFNγ-injected larvae. (A–F) Cell proliferation in control (A, C, E) and IFNγ-injected (B, D, F) larvae. Livers (white outline) were stained with anti-PCNA (red) to label proliferating cells and 2F11 (green) to label cholangiocytes, and were counterstained with DAPI. Representative livers from 3 dpf (A, B), 4 dpf (C, D), and 5 dpf (E, F) are shown. Note that there are fewer cholangiocytes in the IFNγ-injected larvae and fewer proliferating cholangiocytes in the IFNγ-injected at 3 dpf and 4 dpf. This is quantified in Table 1. (G, H) TUNEL staining of whole-mount 5 dpf larvae show no positive cells (white arrows) in the liver (white outline) in either control (G) and IFNγ-injected (H) larvae. Scale bar 25 μm for (A–F).