Abstract
Nuclear microinjection of c-H-ras DNA induced DNA synthesis in reversibly nonproliferating quiescent human cells. The proto-oncogene and oncogene forms were equally effective inducers. In contrast, c-H-ras DNA either alone or in combination with the adenovirus E1A gene did not cause terminally nondividing senescent cells to synthesize DNA.
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- Bernards R., Houweling A., Schrier P. I., Bos J. L., Van der Eb A. J. Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids. Virology. 1982 Jul 30;120(2):422–432. doi: 10.1016/0042-6822(82)90042-3. [DOI] [PubMed] [Google Scholar]
- Bishop J. M. Viral oncogenes. Cell. 1985 Aug;42(1):23–38. doi: 10.1016/s0092-8674(85)80098-2. [DOI] [PubMed] [Google Scholar]
- Burmer G. C., Rabinovitch P. S., Norwood T. H. Evidence for differences in the mechanism of cell cycle arrest between senescent and serum-deprived human fibroblasts: heterokaryon and metabolic inhibitor studies. J Cell Physiol. 1984 Jan;118(1):97–103. doi: 10.1002/jcp.1041180116. [DOI] [PubMed] [Google Scholar]
- Burmer G. C., Zeigler C. J., Norwood T. H. Evidence for endogenous polypeptide-mediated inhibition of cell-cycle transit in human diploid cells. J Cell Biol. 1982 Jul;94(1):187–192. doi: 10.1083/jcb.94.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig R. W., Sager R. Suppression of tumorigenicity in hybrids of normal and oncogene-transformed CHEF cells. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2062–2066. doi: 10.1073/pnas.82.7.2062. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dell'Orco R. T., Mertens J. G., Kruse P. F., Jr Doubling potential, calendar time, and senescence of human diploid cells in culture. Exp Cell Res. 1973 Mar 15;77(1):356–360. doi: 10.1016/0014-4827(73)90588-0. [DOI] [PubMed] [Google Scholar]
- Drescher-Lincoln C. K., Smith J. R. Inhibition of DNA synthesis in senescent-proliferating human cybrids is mediated by endogenous proteins. Exp Cell Res. 1984 Jul;153(1):208–217. doi: 10.1016/0014-4827(84)90462-2. [DOI] [PubMed] [Google Scholar]
- Duthu G. S., Braunschweiger K. I., Pereira-Smith O. M., Norwood T. H., Smith J. R. A long-lived human diploid fibroblast line for cellular aging studies: applications in cell hybridization. Mech Ageing Dev. 1982 Nov;20(3):243–252. doi: 10.1016/0047-6374(82)90091-4. [DOI] [PubMed] [Google Scholar]
- Feramisco J. R., Gross M., Kamata T., Rosenberg M., Sweet R. W. Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells. Cell. 1984 Aug;38(1):109–117. doi: 10.1016/0092-8674(84)90531-2. [DOI] [PubMed] [Google Scholar]
- Gorman S. D., Cristofalo V. J. Reinitiation of cellular DNA synthesis in BrdU-selected nondividing senescent WI-38 cells by simian virus 40 infection. J Cell Physiol. 1985 Oct;125(1):122–126. doi: 10.1002/jcp.1041250116. [DOI] [PubMed] [Google Scholar]
- HAYFLICK L., MOORHEAD P. S. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585–621. doi: 10.1016/0014-4827(61)90192-6. [DOI] [PubMed] [Google Scholar]
- HAYFLICK L. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. Exp Cell Res. 1965 Mar;37:614–636. doi: 10.1016/0014-4827(65)90211-9. [DOI] [PubMed] [Google Scholar]
- Hunter T. Oncogenes and proto-oncogenes: how do they differ? J Natl Cancer Inst. 1984 Oct;73(4):773–786. [PubMed] [Google Scholar]
- Ide T., Tsuji Y., Ishibashi S., Mitsui Y., Toba M. Induction of host DNA synthesis in senescent human diploid fibroblasts by infection with human cytomegalovirus. Mech Ageing Dev. 1984 Apr-May;25(1-2):227–235. doi: 10.1016/0047-6374(84)90143-x. [DOI] [PubMed] [Google Scholar]
- Lacal J. C., Srivastava S. K., Anderson P. S., Aaronson S. A. Ras p21 proteins with high or low GTPase activity can efficiently transform NIH/3T3 cells. Cell. 1986 Feb 28;44(4):609–617. doi: 10.1016/0092-8674(86)90270-9. [DOI] [PubMed] [Google Scholar]
- Land H., Parada L. F., Weinberg R. A. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature. 1983 Aug 18;304(5927):596–602. doi: 10.1038/304596a0. [DOI] [PubMed] [Google Scholar]
- Lanford R. E., Butel J. S. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell. 1984 Jul;37(3):801–813. doi: 10.1016/0092-8674(84)90415-x. [DOI] [PubMed] [Google Scholar]
- Lumpkin C. K., Jr, McClung J. K., Smith J. R. Entry into S phase is inhibited in human fibroblasts by rat liver poly(A)+RNA. Exp Cell Res. 1985 Oct;160(2):544–549. doi: 10.1016/0014-4827(85)90201-0. [DOI] [PubMed] [Google Scholar]
- Mulcahy L. S., Smith M. R., Stacey D. W. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells. Nature. 1985 Jan 17;313(5999):241–243. doi: 10.1038/313241a0. [DOI] [PubMed] [Google Scholar]
- Newbold R. F., Overell R. W. Fibroblast immortality is a prerequisite for transformation by EJ c-Ha-ras oncogene. Nature. 1983 Aug 18;304(5927):648–651. doi: 10.1038/304648a0. [DOI] [PubMed] [Google Scholar]
- Norwood T. H., Pendergrass W. R., Martin G. M. Reinitiation of DNA synthesis in senescent human fibroblasts upon fusion with cells of unlimited growth potential. J Cell Biol. 1975 Mar;64(3):551–556. doi: 10.1083/jcb.64.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pereira-Smith O. M., Fisher S. F., Smith J. R. Senescent and quiescent cell inhibitors of DNA synthesis. Membrane-associated proteins. Exp Cell Res. 1985 Oct;160(2):297–306. doi: 10.1016/0014-4827(85)90177-6. [DOI] [PubMed] [Google Scholar]
- Pereira-Smith O. M., Smith J. R. Evidence for the recessive nature of cellular immortality. Science. 1983 Sep 2;221(4614):964–966. doi: 10.1126/science.6879195. [DOI] [PubMed] [Google Scholar]
- Pereira-Smith O. M., Smith J. R. Expression of SV40 T antigen in finite life-span hybrids of normal and SV40-transformed fibroblasts. Somatic Cell Genet. 1981 Jul;7(4):411–421. doi: 10.1007/BF01542986. [DOI] [PubMed] [Google Scholar]
- Pereira-Smith O. M., Smith J. R. Phenotype of low proliferative potential is dominant in hybrids of normal human fibroblasts. Somatic Cell Genet. 1982 Nov;8(6):731–742. doi: 10.1007/BF01543015. [DOI] [PubMed] [Google Scholar]
- Ruley H. E. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature. 1983 Aug 18;304(5927):602–606. doi: 10.1038/304602a0. [DOI] [PubMed] [Google Scholar]
- Sager R., Tanaka K., Lau C. C., Ebina Y., Anisowicz A. Resistance of human cells to tumorigenesis induced by cloned transforming genes. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7601–7605. doi: 10.1073/pnas.80.24.7601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sefton B. M., Trowbridge I. S., Cooper J. A., Scolnick E. M. The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid. Cell. 1982 Dec;31(2 Pt 1):465–474. doi: 10.1016/0092-8674(82)90139-8. [DOI] [PubMed] [Google Scholar]
- Shih C., Weinberg R. A. Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell. 1982 May;29(1):161–169. doi: 10.1016/0092-8674(82)90100-3. [DOI] [PubMed] [Google Scholar]
- Stein G. H., Yanishevsky R. M. Entry into S phase is inhibited in two immortal cell lines fused to senescent human diploid cells. Exp Cell Res. 1979 Apr;120(1):155–165. doi: 10.1016/0014-4827(79)90546-9. [DOI] [PubMed] [Google Scholar]
- Stein G. H., Yanishevsky R. M., Gordon L., Beeson M. Carcinogen-transformed human cells are inhibited from entry into S phase by fusion to senescent cells but cells transformed by DNA tumor viruses overcome the inhibition. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5287–5291. doi: 10.1073/pnas.79.17.5287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sweet R. W., Yokoyama S., Kamata T., Feramisco J. R., Rosenberg M., Gross M. The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activity. Nature. 1984 Sep 20;311(5983):273–275. doi: 10.1038/311273a0. [DOI] [PubMed] [Google Scholar]
- Taparowsky E., Suard Y., Fasano O., Shimizu K., Goldfarb M., Wigler M. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. Nature. 1982 Dec 23;300(5894):762–765. doi: 10.1038/300762a0. [DOI] [PubMed] [Google Scholar]
- Tsuji Y., Ide T., Ishibashi S. Correlation between the presence of T-antigen and the reinitiation of host DNA synthesis in senescent human diploid fibroblasts after SV40 infection. Exp Cell Res. 1983 Mar;144(1):165–169. doi: 10.1016/0014-4827(83)90450-0. [DOI] [PubMed] [Google Scholar]
- Willingham M. C., Pastan I., Shih T. Y., Scolnick E. M. Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry. Cell. 1980 Apr;19(4):1005–1014. doi: 10.1016/0092-8674(80)90091-4. [DOI] [PubMed] [Google Scholar]