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. 1986 Aug;6(8):3005–3009. doi: 10.1128/mcb.6.8.3005

Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif.

A Hogan, E A Faust
PMCID: PMC367873  PMID: 3023957

Abstract

The mechanism of nonhomologous recombination in murine cells infected with the parvovirus minute virus of mice (MVM) has been investigated by analysis of DNA sequences at recombination junctions in naturally occurring deletion variants of the virus. We report here that nonhomologous recombination in the MVM chromosome is characterized by short homologies, by insertion at recombination junctions of foreign DNA sequences that are enriched for preferred eucaryotic topoisomerase I cleavage sites, and by an association with a common DNA sequence motif of the type 5'-CTATTTCT-3'. Additional analyses of broken MVM chromosomes provided evidence for specific enzymatic cleavage within 5'-CTTATC-3' and 5'-CTATTC-3' sequences. The results indicate that the 5'-CTATTTCT-3' motif is an important genetic element for nonhomologous recombination in the parvovirus chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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