Figure 2. Schematic of the study of collagenase distribution with or without ductal preservation. In the no DI group, after procurement the pancreas was preserved with the two layer method (upper layer: ET-Kyoto solution, lower layer: oxygenated perfluorocarbon). Meanwhile, ET-Kyoto solution was infused through the main pancreatic duct at the procurement site in the DI group before the two layer method. After preservation, collagenase solution with the marking dye was perfused as described in the Methods. Then the pancreas was fixed and analyzed. Dye: a marking dye.