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. 1987 Aug;7(8):3021–3025. doi: 10.1128/mcb.7.8.3021

Plasminogen activator inhibitor type 1 biosynthesis and mRNA level are increased by dexamethasone in human fibrosarcoma cells.

P A Andreasen 1, C Pyke 1, A Riccio 1, P Kristensen 1, L S Nielsen 1, L R Lund 1, F Blasi 1, K Danø 1
PMCID: PMC367928  PMID: 3118190

Abstract

Dexamethasone increases type 1 plasminogen activator inhibitor (PAI-1) activity released from the human fibrosarcoma cell line HT-1080. We demonstrated that dexamethasone caused about 10-fold increases in the intracellular and extracellular levels of PAI-1 protein, as measured by an enzyme-linked immunosorbent assay, in the rate of PAI-1 biosynthesis, and in the PAI-1 mRNA level. The effects on PAI-1 biosynthesis and mRNA level were detectable within 4 h and were maximal 16 to 24 h after the addition of dexamethasone. Cycloheximide did not inhibit the dexamethasone-induced increases in the capacity of the cells to synthesize PAI-1 and in the PAI-1 mRNA level.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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