Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1987 Sep;7(9):3027–3031. doi: 10.1128/mcb.7.9.3027

rRNA genes of Naegleria gruberi are carried exclusively on a 14-kilobase-pair plasmid.

C G Clark 1, G A Cross 1
PMCID: PMC367933  PMID: 2823115

Abstract

An extrachromosomal DNA was discovered in Naegleria gruberi. The 3,000 to 5,000 copies per cell of this 14-kilobase-pair circular plasmid carry all the 18S, 28S, and 5.8S rRNA genes. The presence of the ribosomal DNA of an organism exclusively on a circular extrachromosomal element is without precedent, and Naegleria is only the third eucaryotic genus in which a nuclear plasmid DNA has been found.

Full text

PDF
3027

Images in this article

3028Image
on p.3028
3029Image
on p.3029
3029Image
on p.3029
3030Image
on p.3030

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernards A., Van der Ploeg L. H., Frasch A. C., Borst P., Boothroyd J. C., Coleman S., Cross G. A. Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3' end. Cell. 1981 Dec;27(3 Pt 2):497–505. doi: 10.1016/0092-8674(81)90391-3. [DOI] [PubMed] [Google Scholar]
  2. Cockburn A. F., Taylor W. C., Firtel R. A. Dictyostelium rDNA consists of non-chromosomal palindromic dimers containing 5S and 36S coding regions. Chromosoma. 1978 Dec 21;70(1):19–29. doi: 10.1007/BF00292212. [DOI] [PubMed] [Google Scholar]
  3. Cully D. F., Ip H. S., Cross G. A. Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei. Cell. 1985 Aug;42(1):173–182. doi: 10.1016/s0092-8674(85)80113-6. [DOI] [PubMed] [Google Scholar]
  4. D'Alessio J. M., Harris G. H., Perna P. J., Paule M. R. Ribosomal ribonucleic acid repeat unit of Acanthamoeba castellanii: cloning and restriction endonuclease map. Biochemistry. 1981 Jun 23;20(13):3822–3827. doi: 10.1021/bi00516a024. [DOI] [PubMed] [Google Scholar]
  5. Din N., Engberg J. Extrachromosomal ribosomal RNA genes in Tetrahymena: structure and evolution. J Mol Biol. 1979 Nov 5;134(3):555–574. doi: 10.1016/0022-2836(79)90367-x. [DOI] [PubMed] [Google Scholar]
  6. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  7. Fulton C. Cell differentiation in Naegleria gruberi. Annu Rev Microbiol. 1977;31:597–629. doi: 10.1146/annurev.mi.31.100177.003121. [DOI] [PubMed] [Google Scholar]
  8. Fulton C., Cheng K. L., Lai E. Y. Two calmodulins in Naegleria flagellates: characterization, intracellular segregation, and programmed regulation of mRNA abundance during differentiation. J Cell Biol. 1986 May;102(5):1671–1678. doi: 10.1083/jcb.102.5.1671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fulton C. Macromolecular syntheses during the quick-change act of Naegleria. J Protozool. 1983 May;30(2):192–198. doi: 10.1111/j.1550-7408.1983.tb02902.x. [DOI] [PubMed] [Google Scholar]
  10. Fulton C., Walsh C. Cell differentiation and flagellar elongation in Naegleria gruberi. Dependence on transcription and translation. J Cell Biol. 1980 May;85(2):346–360. doi: 10.1083/jcb.85.2.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  13. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  14. John D. T. Primary amebic meningoencephalitis and the biology of Naegleria fowleri. Annu Rev Microbiol. 1982;36:101–123. doi: 10.1146/annurev.mi.36.100182.000533. [DOI] [PubMed] [Google Scholar]
  15. Johnson P. H., Grossman L. I. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs. Biochemistry. 1977 Sep 20;16(19):4217–4225. doi: 10.1021/bi00638a014. [DOI] [PubMed] [Google Scholar]
  16. Lai E. Y., Walsh C., Wardell D., Fulton C. Programmed appearance of translatable flagellar tubulin mRNA during cell differentiation in Naegleria. Cell. 1979 Aug;17(4):867–878. doi: 10.1016/0092-8674(79)90327-1. [DOI] [PubMed] [Google Scholar]
  17. Levine N. D., Corliss J. O., Cox F. E., Deroux G., Grain J., Honigberg B. M., Leedale G. F., Loeblich A. R., 3rd, Lom J., Lynn D. A newly revised classification of the protozoa. J Protozool. 1980 Feb;27(1):37–58. doi: 10.1111/j.1550-7408.1980.tb04228.x. [DOI] [PubMed] [Google Scholar]
  18. Long E. O., Dawid I. B. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. doi: 10.1146/annurev.bi.49.070180.003455. [DOI] [PubMed] [Google Scholar]
  19. Maly P., Brimacombe R. Refined secondary structure models for the 16S and 23S ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1983 Nov 11;11(21):7263–7286. doi: 10.1093/nar/11.21.7263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mandal R. K. The organization and transcription of eukaryotic ribosomal RNA genes. Prog Nucleic Acid Res Mol Biol. 1984;31:115–160. doi: 10.1016/s0079-6603(08)60376-1. [DOI] [PubMed] [Google Scholar]
  21. Metz B. A., Ward T. E., Welker D. L., Williams K. L. Identification of an endogenous plasmid in Dictyostelium discoideum. EMBO J. 1983;2(4):515–519. doi: 10.1002/j.1460-2075.1983.tb01456.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Molgaard H. V., Matthews H. R., Bradbury E. M. Organisation of genes for ribosomal RNA in Physarum polycephalum. Eur J Biochem. 1976 Sep 15;68(2):541–549. doi: 10.1111/j.1432-1033.1976.tb10842.x. [DOI] [PubMed] [Google Scholar]
  23. Orii H., Suzuki K., Tanaka Y., Yanagisawa K. A new type of plasmid from a wild isolate of Dictyostelium species: the existence of closely situated long inverted repeats. Nucleic Acids Res. 1987 Feb 11;15(3):1097–1107. doi: 10.1093/nar/15.3.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Otsuka T., Nomiyama H., Yoshida H., Kukita T., Kuhara S., Sakaki Y. Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3163–3167. doi: 10.1073/pnas.80.11.3163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rush M. G., Misra R. Extrachromosomal DNA in eucaryotes. Plasmid. 1985 Nov;14(3):177–191. doi: 10.1016/0147-619x(85)90001-0. [DOI] [PubMed] [Google Scholar]
  26. Stevens A. R., Pachler P. F. Discontinuity of 26 s rRNA in Acanthamoeba castellani. J Mol Biol. 1972 May 14;66(2):225–237. doi: 10.1016/0022-2836(72)90475-5. [DOI] [PubMed] [Google Scholar]
  27. Sussman D. J., Lai E. Y., Fulton C. Rapid disappearance of translatable actin mRNA during cell differentiation in Naegleria. J Biol Chem. 1984 Jun 10;259(11):7355–7360. [PubMed] [Google Scholar]
  28. Toh-e A., Araki H., Utatsu I., Oshima Y. Plasmids resembling 2-micrometers DNA in the osmotolerant yeasts Saccharomyces bailii and Saccharomyces bisporus. J Gen Microbiol. 1984 Oct;130(10):2527–2534. doi: 10.1099/00221287-130-10-2527. [DOI] [PubMed] [Google Scholar]
  29. Toh-e A., Tada S., Oshima Y. 2-micrometers DNA-like plasmids in the osmophilic haploid yeast Saccharomyces rouxii. J Bacteriol. 1982 Sep;151(3):1380–1390. doi: 10.1128/jb.151.3.1380-1390.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Vogt V. M., Braun R. Structure of ribosomal DNA in Physarum polycephalum. J Mol Biol. 1976 Sep 25;106(3):567–587. doi: 10.1016/0022-2836(76)90252-7. [DOI] [PubMed] [Google Scholar]
  31. Wild M. A., Gall J. G. An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa. Cell. 1979 Mar;16(3):565–573. doi: 10.1016/0092-8674(79)90030-8. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES