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. 1987 Sep;7(9):3320–3323. doi: 10.1128/mcb.7.9.3320

Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.

P Russell 1, S O Meakin 1, T C Hohman 1, L C Tsui 1, M L Breitman 1
PMCID: PMC367970  PMID: 3313014

Abstract

Although individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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