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. 1987 Sep;7(9):3337–3340. doi: 10.1128/mcb.7.9.3337

Developmental regulation and identification of an isotype encoded by altB, an alpha-tubulin locus in Physarum polycephalum.

L L Green 1, M M Schroeder 1, M A Diggins 1, W F Dove 1
PMCID: PMC367974  PMID: 2823127

Abstract

A subcloned portion of the 5' nontranslated sequence from a Physarum alpha-tubulin cDNA is specific for a single alpha-tubulin locus, altB, of Physarum polycephalum. We find that this locus is expressed only in the plasmodium and encodes at least an alpha 1-tubulin isotype, which we have designated alpha 1B. Hybridization patterns of other subclones of this cDNA reveal two sequences for alpha-tubulin at the altB locus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birkett C. R., Foster K. E., Johnson L., Gull K. Use of monoclonal antibodies to analyse the expression of a multi-tubulin family. FEBS Lett. 1985 Aug 5;187(2):211–218. doi: 10.1016/0014-5793(85)81244-8. [DOI] [PubMed] [Google Scholar]
  2. Burland T. G., Gull K., Schedl T., Boston R. S., Dove W. F. Cell type-dependent expression of tubulins in Physarum. J Cell Biol. 1983 Dec;97(6):1852–1859. doi: 10.1083/jcb.97.6.1852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burland T. G., Schedl T., Gull K., Dove W. F. Genetic analysis of resistance to benzimidazoles in Physarum: differential expression of beta-tubulin genes. Genetics. 1984 Sep;108(1):123–141. doi: 10.1093/genetics/108.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diggins M. A., Dove W. F. Distribution of acetylated alpha-tubulin in Physarum polycephalum. J Cell Biol. 1987 Feb;104(2):303–309. doi: 10.1083/jcb.104.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elliott E. M., Henderson G., Sarangi F., Ling V. Complete sequence of three alpha-tubulin cDNAs in Chinese hamster ovary cells: each encodes a distinct alpha-tubulin isoprotein. Mol Cell Biol. 1986 Mar;6(3):906–913. doi: 10.1128/mcb.6.3.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green L. L., Dove W. F. Tubulin proteins and RNA during the myxamoeba-flagellate transformation of Physarum polycephalum. Mol Cell Biol. 1984 Sep;4(9):1706–1711. doi: 10.1128/mcb.4.9.1706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Havercroft J. C., Gull K. Demonstration of different patterns of microtubule organization in Physarum polycephalum myxamoebae and plasmodia using immunofluorescence microscopy. Eur J Cell Biol. 1983 Nov;32(1):67–74. [PubMed] [Google Scholar]
  8. Kilmartin J. V., Wright B., Milstein C. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982 Jun;93(3):576–582. doi: 10.1083/jcb.93.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krämmer G., Singhofer-Wowra M., Seedorf K., Little M., Schedl T. A plasmodial alpha-tubulin cDNA from Physarum polycephalum. Nucleotide sequence and comparative analysis. J Mol Biol. 1985 Jun 25;183(4):633–638. doi: 10.1016/0022-2836(85)90176-7. [DOI] [PubMed] [Google Scholar]
  10. Marsh J. L., Erfle M., Wykes E. J. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984 Dec;32(3):481–485. doi: 10.1016/0378-1119(84)90022-2. [DOI] [PubMed] [Google Scholar]
  11. Paul E. C., Roobol A., Foster K. E., Gull K. Patterns of tubulin isotype synthesis and usage during mitotic spindle morphogenesis in Physarum. Cell Motil Cytoskeleton. 1987;7(3):272–281. doi: 10.1002/cm.970070309. [DOI] [PubMed] [Google Scholar]
  12. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  13. Roobol A., Wilcox M., Paul E. C., Gull K. Identification of tubulin isoforms in the plasmodium of Physarum polycephalum by in vitro microtubule assembly. Eur J Cell Biol. 1984 Jan;33(1):24–28. [PubMed] [Google Scholar]
  14. Sasse R., Glyn M. C., Birkett C. R., Gull K. Acetylated alpha-tubulin in Physarum: immunological characterization of the isotype and its usage in particular microtubular organelles. J Cell Biol. 1987 Jan;104(1):41–49. doi: 10.1083/jcb.104.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schedl T., Burland T. G., Gull K., Dove W. F. Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum. J Cell Biol. 1984 Jul;99(1 Pt 1):155–165. doi: 10.1083/jcb.99.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schmitt J. J., Cohen B. N. Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography. Anal Biochem. 1983 Sep;133(2):462–464. doi: 10.1016/0003-2697(83)90109-4. [DOI] [PubMed] [Google Scholar]
  17. Silflow C. D., Chisholm R. L., Conner T. W., Ranum L. P. The two alpha-tubulin genes of Chlamydomonas reinhardi code for slightly different proteins. Mol Cell Biol. 1985 Sep;5(9):2389–2398. doi: 10.1128/mcb.5.9.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Singhofer-Wowra M., Little M., Clayton L., Dawson P., Gull K. Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum. J Mol Biol. 1986 Dec 20;192(4):919–924. doi: 10.1016/0022-2836(86)90037-9. [DOI] [PubMed] [Google Scholar]
  19. Sullivan K. F., Lau J. T., Cleveland D. W. Apparent gene conversion between beta-tubulin genes yields multiple regulatory pathways for a single beta-tubulin polypeptide isotype. Mol Cell Biol. 1985 Sep;5(9):2454–2465. doi: 10.1128/mcb.5.9.2454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Theurkauf W. E., Baum H., Bo J., Wensink P. C. Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8477–8481. doi: 10.1073/pnas.83.22.8477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Villasante A., Wang D., Dobner P., Dolph P., Lewis S. A., Cowan N. J. Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes. Mol Cell Biol. 1986 Jul;6(7):2409–2419. doi: 10.1128/mcb.6.7.2409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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