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. 1987 Oct;7(10):3482–3489. doi: 10.1128/mcb.7.10.3482

Purification of the c-fos enhancer-binding protein.

R Prywes 1, R G Roeder 1
PMCID: PMC368000  PMID: 3316979

Abstract

We have purified the c-fos enhancer-binding protein from HeLa cell nuclear extracts. The key purification steps involved chromatography on a nonspecific DNA affinity column, from which binding activity and other protein were eluted at low salt concentrations, followed by chromatography on a specific oligonucleotide affinity column, from which the enhancer binding activity was specifically eluted at high salt concentrations. The purified protein had a strong affinity for the c-fos enhancer dyad symmetry sequence, with an equilibrium dissociation constant of 3.3 x 10(-11) M. This affinity was at least 50,000-fold stronger than that found for nonspecific DNA sequences.

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Selected References

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