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Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1987 Oct;7(10):3548–3553. doi: 10.1128/mcb.7.10.3548

An embryonic DNA-binding protein specific for the promoter of the retrovirus long terminal repeat.

F Flamant 1, C C Gurin 1, J A Sorge 1
PMCID: PMC368007  PMID: 2824991

Abstract

Retrovirus expression is restricted in embryonal carcinoma (EC) cells but not in many differentiated cell lines. We used a very sensitive gel retardation assay to detect sequence-specific DNA-binding proteins in crude nuclear extracts obtained from EC and differentiated cells. Four binding sites were mapped in the noncoding sequences of the amphotropic murine leukemia virus. Strong binding to the CCAAT consensus sequence located in the promoter was specifically observed with EC nuclear extract. The binding protein is called EPBF (embryonal promoter-binding factor), and it is a candidate for the repressor of retrovirus transcription.

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Selected References

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