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. 1987 Oct;7(10):3836–3841. doi: 10.1128/mcb.7.10.3836

Murine Hox-1.7 homeo-box gene: cloning, chromosomal location, and expression.

M R Rubin 1, W King 1, L E Toth 1, I S Sawczuk 1, M S Levine 1, P D'Eustachio 1, M C Nguyen-Huu 1
PMCID: PMC368043  PMID: 2891029

Abstract

A new murine homeo-box, called Hox-1.7, has been identified in a rare cDNA from F9 teratocarcinoma stem cells. The Hox-1.7 homeo-box is 68 and 72% homologous to the Drosophila antennapedia (Antp) and iab-7 homeo-boxes, respectively. A major 2.5-kilobase transcript and several minor transcripts were detected by Northern blot (RNA blot) analysis in adult tissues as well as in midgestational embryos. The posterior spinal cord was found to be a major site of Hox-1.7 expression in 12.5-day-old embryos. Somatic cell hybrids were used to map the Hox-1.7 gene to mouse chromosome 6. Restriction fragment length polymorphisms associated with either the Hox-1.7 gene or the previously known Hox-1 complex were identified. Their distribution patterns in recombinant inbred mouse strains were used to determine the linkage between the two loci as well as to other loci on chromosome 6. This maps Hox-1 and Hox-1.7 close to two mouse loci that affect morphogenesis, postaxial hemimelia (px) and hypodactyly (Hd).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awgulewitsch A., Utset M. F., Hart C. P., McGinnis W., Ruddle F. H. Spatial restriction in expression of a mouse homoeo box locus within the central nervous system. 1986 Mar 27-Apr 2Nature. 320(6060):328–335. doi: 10.1038/320328a0. [DOI] [PubMed] [Google Scholar]
  2. Beachy P. A., Helfand S. L., Hogness D. S. Segmental distribution of bithorax complex proteins during Drosophila development. Nature. 1985 Feb 14;313(6003):545–551. doi: 10.1038/313545a0. [DOI] [PubMed] [Google Scholar]
  3. Bender W., Akam M., Karch F., Beachy P. A., Peifer M., Spierer P., Lewis E. B., Hogness D. S. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):23–29. doi: 10.1126/science.221.4605.23. [DOI] [PubMed] [Google Scholar]
  4. Berstine E. G., Hooper M. L., Grandchamp S., Ephrussi B. Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899–3903. doi: 10.1073/pnas.70.12.3899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Breier G., Bućan M., Francke U., Colberg-Poley A. M., Gruss P. Sequential expression of murine homeo box genes during F9 EC cell differentiation. EMBO J. 1986 Sep;5(9):2209–2215. doi: 10.1002/j.1460-2075.1986.tb04486.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bućan M., Yang-Feng T., Colberg-Poley A. M., Wolgemuth D. J., Guenet J. L., Francke U., Lehrach H. Genetic and cytogenetic localisation of the homeo box containing genes on mouse chromosome 6 and human chromosome 7. EMBO J. 1986 Nov;5(11):2899–2905. doi: 10.1002/j.1460-2075.1986.tb04585.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carroll S. B., Scott M. P. Localization of the fushi tarazu protein during Drosophila embryogenesis. Cell. 1985 Nov;43(1):47–57. doi: 10.1016/0092-8674(85)90011-x. [DOI] [PubMed] [Google Scholar]
  8. Colberg-Poley A. M., Voss S. D., Chowdhury K., Gruss P. Structural analysis of murine genes containing homoeo box sequences and their expression in embryonal carcinoma cells. 1985 Apr 25-May 1Nature. 314(6013):713–718. doi: 10.1038/314713a0. [DOI] [PubMed] [Google Scholar]
  9. Colberg-Poley A. M., Voss S. D., Chowdhury K., Stewart C. L., Wagner E. F., Gruss P. Clustered homeo boxes are differentially expressed during murine development. Cell. 1985 Nov;43(1):39–45. doi: 10.1016/0092-8674(85)90010-8. [DOI] [PubMed] [Google Scholar]
  10. D'Eustachio P., Owens G. C., Edelman G. M., Cunningham B. A. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7631–7635. doi: 10.1073/pnas.82.22.7631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. D'Hoostelaere L. A., Jouvin-Marche E., Huppi K. Localization of CT beta and C kappa on mouse chromosome 6. Immunogenetics. 1985;22(3):277–283. doi: 10.1007/BF00404487. [DOI] [PubMed] [Google Scholar]
  12. Deschamps J., de Laaf R., Joosen L., Meijlink F., Destrée O. Abundant expression of homeobox genes in mouse embryonal carcinoma cells correlates with chemically induced differentiation. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1304–1308. doi: 10.1073/pnas.84.5.1304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. DiNardo S., Kuner J. M., Theis J., O'Farrell P. H. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell. 1985 Nov;43(1):59–69. doi: 10.1016/0092-8674(85)90012-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Duboule D., Baron A., Mähl P., Galliot B. A new homeo-box is present in overlapping cosmid clones which define the mouse Hox-1 locus. EMBO J. 1986 Aug;5(8):1973–1980. doi: 10.1002/j.1460-2075.1986.tb04452.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fjose A., McGinnis W. J., Gehring W. J. Isolation of a homoeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts. Nature. 1985 Jan 24;313(6000):284–289. doi: 10.1038/313284a0. [DOI] [PubMed] [Google Scholar]
  16. Garber R. L., Kuroiwa A., Gehring W. J. Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila. EMBO J. 1983;2(11):2027–2036. doi: 10.1002/j.1460-2075.1983.tb01696.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gaunt S. J., Miller J. R., Powell D. J., Duboule D. Homoeobox gene expression in mouse embryos varies with position by the primitive streak stage. Nature. 1986 Dec 18;324(6098):662–664. doi: 10.1038/324662a0. [DOI] [PubMed] [Google Scholar]
  18. Harding K., Wedeen C., McGinnis W., Levine M. Spatially regulated expression of homeotic genes in Drosophila. Science. 1985 Sep 20;229(4719):1236–1242. doi: 10.1126/science.3898362. [DOI] [PubMed] [Google Scholar]
  19. Hart C. P., Awgulewitsch A., Fainsod A., McGinnis W., Ruddle F. H. Homeo box gene complex on mouse chromosome 11: molecular cloning, expression in embryogenesis, and homology to a human homeo box locus. Cell. 1985 Nov;43(1):9–18. doi: 10.1016/0092-8674(85)90007-8. [DOI] [PubMed] [Google Scholar]
  20. Hauser C. A., Joyner A. L., Klein R. D., Learned T. K., Martin G. R., Tjian R. Expression of homologous homeo-box-containing genes in differentiated human teratocarcinoma cells and mouse embryos. Cell. 1985 Nov;43(1):19–28. doi: 10.1016/0092-8674(85)90008-x. [DOI] [PubMed] [Google Scholar]
  21. Hummel K. P. Hypodactyly, a semidominant lethal mutation in mice. J Hered. 1970 Sep-Oct;61(5):219–220. doi: 10.1093/oxfordjournals.jhered.a108088. [DOI] [PubMed] [Google Scholar]
  22. Jackson I. J., Schofield P., Hogan B. A mouse homoeo box gene is expressed during embryogenesis and in adult kidney. Nature. 1985 Oct 24;317(6039):745–748. doi: 10.1038/317745a0. [DOI] [PubMed] [Google Scholar]
  23. Joyner A. L., Kornberg T., Coleman K. G., Cox D. R., Martin G. R. Expression during embryogenesis of a mouse gene with sequence homology to the Drosophila engrailed gene. Cell. 1985 Nov;43(1):29–37. doi: 10.1016/0092-8674(85)90009-1. [DOI] [PubMed] [Google Scholar]
  24. Joyner A. L., Martin G. R. En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis. Genes Dev. 1987 Mar;1(1):29–38. doi: 10.1101/gad.1.1.29. [DOI] [PubMed] [Google Scholar]
  25. Krumlauf R., Holland P. W., McVey J. H., Hogan B. L. Developmental and spatial patterns of expression of the mouse homeobox gene, Hox 2.1. Development. 1987 Apr;99(4):603–617. doi: 10.1242/dev.99.4.603. [DOI] [PubMed] [Google Scholar]
  26. Laughon A., Scott M. P. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature. 1984 Jul 5;310(5972):25–31. doi: 10.1038/310025a0. [DOI] [PubMed] [Google Scholar]
  27. Levine M., Rubin G. M., Tjian R. Human DNA sequences homologous to a protein coding region conserved between homeotic genes of Drosophila. Cell. 1984 Oct;38(3):667–673. doi: 10.1016/0092-8674(84)90261-7. [DOI] [PubMed] [Google Scholar]
  28. Lewis E. B. A gene complex controlling segmentation in Drosophila. Nature. 1978 Dec 7;276(5688):565–570. doi: 10.1038/276565a0. [DOI] [PubMed] [Google Scholar]
  29. Manley J. L., Levine M. S. The homeo box and mammalian development. Cell. 1985 Nov;43(1):1–2. doi: 10.1016/0092-8674(85)90002-9. [DOI] [PubMed] [Google Scholar]
  30. Martin G. R. Nomenclature for homoeobox-containing genes. Nature. 1987 Jan 1;325(6099):21–22. doi: 10.1038/325021b0. [DOI] [PubMed] [Google Scholar]
  31. Mavilio F., Simeone A., Giampaolo A., Faiella A., Zappavigna V., Acampora D., Poiana G., Russo G., Peschle C., Boncinelli E. Differential and stage-related expression in embryonic tissues of a new human homoeobox gene. Nature. 1986 Dec 18;324(6098):664–668. doi: 10.1038/324664a0. [DOI] [PubMed] [Google Scholar]
  32. McGinnis W., Garber R. L., Wirz J., Kuroiwa A., Gehring W. J. A homologous protein-coding sequence in Drosophila homeotic genes and its conservation in other metazoans. Cell. 1984 Jun;37(2):403–408. doi: 10.1016/0092-8674(84)90370-2. [DOI] [PubMed] [Google Scholar]
  33. McGinnis W., Hart C. P., Gehring W. J., Ruddle F. H. Molecular cloning and chromosome mapping of a mouse DNA sequence homologous to homeotic genes of Drosophila. Cell. 1984 Oct;38(3):675–680. doi: 10.1016/0092-8674(84)90262-9. [DOI] [PubMed] [Google Scholar]
  34. McGinnis W., Levine M. S., Hafen E., Kuroiwa A., Gehring W. J. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes. 1984 Mar 29-Apr 4Nature. 308(5958):428–433. doi: 10.1038/308428a0. [DOI] [PubMed] [Google Scholar]
  35. Nüsslein-Volhard C., Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. doi: 10.1038/287795a0. [DOI] [PubMed] [Google Scholar]
  36. Ouweneel W. J. Developmental genetics of homoeosis. Adv Genet. 1976;18:179–248. doi: 10.1016/s0065-2660(08)60439-3. [DOI] [PubMed] [Google Scholar]
  37. Poole S. J., Kauvar L. M., Drees B., Kornberg T. The engrailed locus of Drosophila: structural analysis of an embryonic transcript. Cell. 1985 Jan;40(1):37–43. doi: 10.1016/0092-8674(85)90306-x. [DOI] [PubMed] [Google Scholar]
  38. Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Homeo box genes of the Antennapedia and bithorax complexes of Drosophila. Cell. 1985 Nov;43(1):71–80. doi: 10.1016/0092-8674(85)90013-3. [DOI] [PubMed] [Google Scholar]
  39. Rubin M. R., Toth L. E., Patel M. D., D'Eustachio P., Nguyen-Huu M. C. A mouse homeo box gene is expressed in spermatocytes and embryos. Science. 1986 Aug 8;233(4764):663–667. doi: 10.1126/science.3726554. [DOI] [PubMed] [Google Scholar]
  40. Scott M. P., Weiner A. J. Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115–4119. doi: 10.1073/pnas.81.13.4115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Silver J. Confidence limits for estimates of gene linkage based on analysis of recombinant inbred strains. J Hered. 1985 Nov-Dec;76(6):436–440. doi: 10.1093/oxfordjournals.jhered.a110140. [DOI] [PubMed] [Google Scholar]
  42. Simeone A., Mavilio F., Bottero L., Giampaolo A., Russo G., Faiella A., Boncinelli E., Peschle C. A human homoeo box gene specifically expressed in spinal cord during embryonic development. Nature. 1986 Apr 24;320(6064):763–765. doi: 10.1038/320763a0. [DOI] [PubMed] [Google Scholar]
  43. Stevens L. C. The biology of teratomas. Adv Morphog. 1967;6:1–31. doi: 10.1016/b978-1-4831-9953-5.50005-6. [DOI] [PubMed] [Google Scholar]
  44. Strickland S., Smith K. K., Marotti K. R. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell. 1980 Sep;21(2):347–355. doi: 10.1016/0092-8674(80)90471-7. [DOI] [PubMed] [Google Scholar]
  45. Tsonis P. A., Adamson E. D. Specific expression of homoeobox-containing genes during induced differentiation of embryonal carcinoma cells. Biochem Biophys Res Commun. 1986 May 29;137(1):520–527. doi: 10.1016/0006-291x(86)91241-6. [DOI] [PubMed] [Google Scholar]
  46. Tulchin N., Taylor B. A. gamma-Glutamyl cyclotransferase: a new genetic polymorphism in the mouse (Mus musculus) linked to Lyt-2. Genetics. 1981 Sep;99(1):109–116. doi: 10.1093/genetics/99.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Utset M. F., Awgulewitsch A., Ruddle F. H., McGinnis W. Region-specific expression of two mouse homeo box genes. Science. 1987 Mar 13;235(4794):1379–1382. doi: 10.1126/science.2881353. [DOI] [PubMed] [Google Scholar]
  48. Wolgemuth D. J., Engelmyer E., Duggal R. N., Gizang-Ginsberg E., Mutter G. L., Ponzetto C., Viviano C., Zakeri Z. F. Isolation of a mouse cDNA coding for a developmentally regulated, testis-specific transcript containing homeo box homology. EMBO J. 1986 Jun;5(6):1229–1235. doi: 10.1002/j.1460-2075.1986.tb04351.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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