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. 1987 Nov;7(11):3916–3922. doi: 10.1128/mcb.7.11.3916

Differential methylation of the ornithine carbamoyl transferase gene on active and inactive mouse X chromosomes.

L J Mullins 1, G Veres 1, C T Caskey 1, V Chapman 1
PMCID: PMC368059  PMID: 2828919

Abstract

Ornithine carbamoyl transferase (Oct) is an X-linked gene which exhibits tissue-specific expression. To determine whether methylation of specific CpG sequences plays a role in dosage compensation or tissue-specific expression of the gene, 13 potentially methylatable sites were identified over a 30-kilobase (kb) region spanning from approximately 15 kb upstream to beyond exon II. Fragments of the Mus hortulanus Oct gene were used as probes to establish the degree of methylation at each site. By considering the methylation status in liver (expressing tissue) versus kidney (nonexpressing tissue) from male and female mice, the active and inactive genes could be investigated on active and inactive X-chromosome backgrounds. One MspI site, 12 kb 5' of the Oct-coding region, was cleaved by HpaII in liver DNA from males but not in kidney DNA from males and thus exhibited complete correlation with tissue-specific expression of the gene. Six other sites showed partial methylation, reflecting incomplete correlation with tissue-specific expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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