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Journal of Interferon & Cytokine Research logoLink to Journal of Interferon & Cytokine Research
. 2012 Mar;32(3):103–110. doi: 10.1089/jir.2011.0099

The Role of Signal Transducer and Activator of Transcription-2 in the Interferon Response

Håkan C Steen 1, Ana M Gamero 1,
PMCID: PMC3680977  PMID: 22280068

Abstract

The signal transducer and activator of transcription-2 (STAT2) was discovered as a cellular component of the DNA binding complex known as interferon (IFN) stimulated gene factor-3. Numerous studies have confirmed that STAT2 operates as a positive regulator in the transcriptional activation response elicited by IFNs. In this article, we highlight the progress made in elucidating the pivotal role of STAT2 in driving the expression of IFN-induced genes, innate antiviral immunity, apoptosis, and cancer. A better understanding of the functional role of STAT2 in the IFN response and how STAT2 is regulated will uncover new clues to its role in diseases.

Introduction

Interferons (IFNs) constitute a family of multifunctional cytokines best described for their role in host defense by linking adaptive and innate immunity and exerting antiviral, antiproliferative, apoptotic, and antitumor effects. IFNs are classified into 3 types: type I (IFN-α/β), type II (IFN-γ), and type III [IFN-λ/interleukin (IL)28/IL29] (reviewed in Platanias 2005; Donnelly and Kotenko 2010). The biological responses elicited by IFNs are mediated by the transcription of multiple IFN induced genes, which is primarily dependent on the activation and nuclear translocation of signal transducer and activator of transcription (STAT) factors. IFNs activate the Janus kinase (JAK)/STAT pathway by binding to their corresponding receptor complex (IFNAR1 and IFNAR2 for type I IFNs; IFNGR1 and IFNGR2 for type II IFN; and IFNLR1 and IL10R2 for type III IFNs). STAT2 and STAT1 become activated in response to all 3 types of IFNs. JAK1 and TYK2 are preassociated with the type I and type III IFN receptor, and phosphorylate specific tyrosine residues within the receptor chains, which serve as docking sites for the recruitment of STATs (Yan and others 1996). JAKs phosphorylate a conserved tyrosine (Y) residue situated in the C-terminal region of STAT2 (Y690 in human and Y689 in mouse) and STAT1 (Y701) that, in turn, allows for STAT1 and STAT2 to interact via reciprocal SH2-phosphotyrosyl interactions (Fu 1992; Shuai and others 1993, 1994; Improta and others 1994;). To this date, STAT2 has only been found to be activated by IFNs and urokinase (Qureshi and others 1996; Dumler and others 1999). Formation of the interferon stimulated gene factor-3 (ISGF3) complex takes place when activated STAT1:STAT2 heterodimers are released from the receptor chains to bind the DNA-binding adapter protein, IRF9. ISGF3 translocates to the nucleus and binds DNA containing an IFN-stimulated response element (ISRE) to activate gene transcription of IFN-stimulated genes (ISGs) (Kessler and others 1990; Veals and others 1993). While STAT1 becomes serine phosphorylated in the chromatin to acquire maximal transcriptional activity, it is unknown whether serine phosphorylation also occurs in STAT2 (Wen and others 1995).

Multiple studies conducted in cell lines and in animal models demonstrate the multifaceted role of STAT2 in various biological systems. In this article, the following features of STAT2 will be covered: (i) STAT2 is vital in innate immunity; (ii) specific viruses preferentially target STAT2 to disarm the IFN antiviral response; (iii) STAT2 has dual roles as a transcriptional repressor and transcriptional activator; and (iv) STAT2 is critical in promoting IFN-induced apoptosis. Further, accumulating evidence now supports the existence of alternative STAT2 signaling pathways that are independent of STAT1.

STAT2 and Structural Domains

STAT2 was discovered as a 113 kDa protein, the largest polypeptide in the ISGF3 complex (Fu and others 1990). STAT2 is 1 of the 7 members of the STAT family as the product of the STAT2 gene mapped to human chromosome 12 and mouse chromosome 10. While it predominantly resides in the cytoplasm, nucleocytoplasmic shuttling of STAT2 occurs in the absence of IFN stimulation (Banninger and Reich 2004). Human and murine STAT2 are highly homologous (76% identity over the first 712 amino acids), but they diverge substantially at the carboxy terminus (Park and others 1999). The transactivation domain (TAD) of mouse STAT2 is longer and contains sequences with no homology to other known proteins (Farrar and others 2000). The STAT2 gene product contains 6 domains: an N-terminal domain (NTD), a coiled-coil domain (CC), a DNA-binding DNA (DBD), a linker domain (LD), a Src homology-2 domain (SH2), and a TAD. These 6 domains are conserved in all STATs.

The role of STAT2 NTD is not well defined, but various reports indicate its requirement for the tyrosine phosphorylation of STAT2 in response to type I IFNs (Qureshi and others 1996); binding of STAT2 to IFNAR2 (Li and others 1997); and cooperative binding of STAT1:STAT2 heterodimers or ISGF3 to promoters that contain tandem IFN-γ activation sequences (GAS) or ISRE elements, respectively (Li and others 1998). In addition, BRG1, a key component of the chromatin remodeling complex SWI2-SNF2, can bind to the STAT2 NTD and facilitate transcription of the ISGs IFITM1 and IFI27 (Huang and others 2002). STAT2 CC mediates protein interactions and is the domain IRF9 binds (Martinez-Moczygemba and others 1997). STAT2 DBD was described as not binding DNA as a part of ISGF3, as direct binding of ISGF3 to DNA was mediated by STAT1 and IRF9 (Bluyssen and Levy 1997). However, other studies have reported indirect evidence that STAT2 binds DNA as a part of an ISGF3-independent STAT2-containing signaling complex (Ghislain and Fish 1996; Ghislain and others 2001; Brierley and Fish 2005). Another characteristic of STAT2 DBD is that it contains a stretch of basic residues that functions as a bipartite nuclear localization signal (NLS) when paired together with the corresponding region in STAT1 after ISGF3 formation (Melen and others 2001). No function for STAT2 LD is known. STAT2 SH2 serves 2 main functions: binding to phosphorylated IFNAR1, thereby making STAT2 available for Tyk2-mediated tyrosine phosphorylation; and binding to tyrosine phosphorylated STAT1 (pSTAT1) to form an active heterodimer (Colamonici and others 1994; Gupta and others 1996; Yan and others 1996). The most C-terminal domain is the TAD, which is essential for the recruitment of transcriptional regulators. Within the STAT2 TAD lies the nuclear export signal (NES) (Banninger and Reich 2004). This leucine-rich region allows for interactions between unphosphorylated STAT2 and Crm1, which is an integral protein of the nuclear export machinery. Furthermore, nucleocytoplasmic shuttling of STAT2 is attributed to the constitutive binding of STAT2 to the NLS-containing IRF9 to transport STAT2 into the nucleus, while the STAT2 NES exports STAT2 back to the cytosol (Martinez-Moczygemba and others 1997; Lau and others 2000; Banninger and Reich 2004).

The protein structures of STAT2 and STAT2:STAT1 heterodimers remain to be solved, but based on solved STAT1 structures and biochemical data it has been demonstrated that STAT2 and STAT1 already co-exist as heterodimers in resting cells (Stancato and others 1996). The STAT dimer binds to each other in an antiparallel conformation through interactions between their NTDs and through reciprocal CC and DBD interactions (Mao and others 2005). IFN stimulation promotes a structural rearrangement in which tyrosine-phosphorylation of the 2 STATs is a critical step for the re-orientation from antiparallel to parallel conformation (Chen and others 1998).

IFN-Induced STAT2-Containing Transcriptional Complexes

In addition to ISGF3, multiple type I IFN-activated STAT2-containing signaling complexes can form. For instance, the IRF9-independent STAT2:STAT1 complex was identified to bind with low affinity to a DNA-sequence similar to the GAS element (Ghislain and Fish 1996). Double mutations introduced in the STAT2 DBD (Valine 453, 454 to Isoleucine) impaired the anti-viral response of IFN-α-treated fibrosarcoma cells to encephalomyocarditis virus infection (Brierley and Fish 2005). Later studies determined that type I IFN-induced STAT2:STAT1 heterodimers stimulated the expression of a subset of IFN-sensitive genes, whose promoters contained a GAS element, which was highly dependent on STAT2 DBD (Ghislain and others 2001; Brierley and others 2006; Jia and others 2007). These findings demonstrated that STAT2 DBD is functional in IFN-induced transcriptional responses.

Other identified STAT2-containing IFN-α regulated complexes include STAT2:STAT3 heterodimers found to exist in human myeloma U266 cells (Ghislain and Fish 1996) and STAT2:STAT6 heterodimers (Gupta and others 1999), the latter being cell type specific. It is unknown whether the STAT2 containing heterodimers just described bind DNA and are transcriptionally active. However, STAT2 did inhibit IL-4 signaling through its binding to STAT6, thereby preventing STAT6 homodimer formation (Kim and Lee 2011). Interestingly, STAT2 has been shown to be constitutively associated with IRF9 independently of STAT1 (Martinez-Moczygemba and others 1997). STAT2:IRF9 heterodimers weakly bind DNA and mediate IFN-stimulated gene expression (Bluyssen and Levy 1997). They are transcriptionally functional via ISRE elements in STAT1 deficient or STAT1 knockdown cells in a cell-type specific manner (Sarkis and others 2006; Lou and others 2009).

It has been known for some time that type II IFN (IFN-γ) activates ISGF3 in murine cells with no evidence that this phenomenon occurs in human cells (Matsumoto and others 1999; Zimmermann and others 2005). A recent report, however, found for the first time that IFN-γ activates a novel ISGF3 complex termed (ISGF3II) in human cells (Morrow and others 2011). What distinguishes ISGF3II from the classical ISGF3 is the presence of unphosphorylated STAT2 associated with STAT1 and IRF9. Another difference is the low mRNA levels of ISGF3II-dependent ISG gene expression (EIF2AK2 and IFIT3) induced by IFN-γ; which required longer exposure to IFN-γ (at least 24 h) to be detected when compared with the levels achieved with IFN-α activated ISGF3. ISGF3II is functional, as it mediated the antiviral response to IFN-γ. Figure 1 shows STAT2 containing complexes induced by each type of IFNs and the IFN responsive element they recognize.

FIG. 1.

FIG. 1.

Composition of multiple signal transducer and activator of transcription-2 (STAT2) containing transcriptional complexes induced by interferons (IFNs). STAT2 can associate with STAT1 and IRF9 to form interferon stimulated gene factor-3 (ISGF3) or ISGF3II (in human cells). In addition, STAT2 can form heterodimers individually with either STAT1 or IRF9. Each of these complexes will bind IFN-stimulated response element (ISRE) or IFN-γ activation sequences (GAS). Not represented are STAT2:STAT3 heterodimers and STAT2:STAT6 heterodimers. Tyrosine phosphorylated STAT1 and STAT2 are marked with P.

STAT2 as a Component of the IFN-Induced Transcriptional Machinery

STAT2 assists in transcriptional induction by providing its TAD to recruit histone acetyltransferases (HATs), including GCN5 and p300/CREB binding protein (CBP) (Bhattacharya and others 1996; Paulson and others 2002). Other molecules such as the tata-box-binding protein component TAFII130 and DRIP150, a subunit of the multimeric Mediator coactivator complex, also interact with STAT2 and are important in ISGF3-dependent gene transcription (Paulson and others 2002; Lau and others 2003). Furthermore, histone deacetylases (HDACs) also contribute positively to IFN-regulated gene expression (Nusinzon and Horvath 2003; Chang and others 2004). IFN-α promotes histone H4 deacetylation, which depends on STAT2 interacting with HDAC1 after IFN-α treatment. STAT2 binding to HDAC1 occurs in the nucleus. Most recently, pp32, a component of the inhibitor of acetyltransferase complex, was identified as another positive regulator of type I IFN induced ISG transcription (Kadota and Nagata 2011). Although pp32 was not required in the tyrosine phosphorylation or nuclear localization of STAT2 and STAT1, its association with STAT2 and STAT1 in an IFN-dependent manner was needed to enhance expression of ISGs. Knockdown of pp32, however, was not sufficient to completely suppress IFN-induced gene transcription, indicating that additional STAT2-interacting proteins can make up for the loss of pp32. Figure 2 shows proteins identified up to now to associate with STAT2 and the site of interaction if known.

FIG. 2.

FIG. 2.

Structural organization of STAT2 and identified interacting proteins. STAT2 contains 6 main domains: N-terminal domain (NTD); Coiled-coil domain (C-C); DNA binding domain (DBD); Linker domain (LD); Src-homology domain-2 (SH2); and Transactivation domain (TAD). Shown are key post-translational modification sites: tyrosine (Y) phosphorylation of Y690 in humans and Y689 in mice; and acetylation of lysine (K)390 in humans. Nine proteins associate with STAT2, of which only 5 interaction sites have been mapped. Protein-protein interactions are not drawn to scale.

As mentioned earlier, STAT2 shuttles between the cytosol and the nucleus in resting cells (Banninger and Reich 2004). The functional relevance of nuclear STAT2 in unstimulated cells remains undefined, but a recent study sheds new light on the role of unphosphorylated STAT2 in the nucleus. Testoni and others (2011b) performed chromatin immunoprecitation (ChIP)-chip assay with anti-STAT2 antibodies on Huh7-liver cells and primary hepatocytes to evaluate changes in STAT2-binding to chromatin in response to IFN-α. Surprisingly, several ISG promoters (70 out of 113 studied) were occupied by STAT2 before IFN-α treatment. Out of the 70 promoters that had STAT2 bound before treatment, 10 were no longer occupied with STAT2 after treatment. These 2 findings revealed the existence of a nonclassical STAT2 signaling pathway. Parallel ChIP-assays conducted with anti-phosphotyrosine-STAT2 antibodies distinguished between unphosphorylated STAT2 and tyrosine-pSTAT2 bound to the ISG promoters. As predicted, the majority of promoters that gained STAT2 in response to IFN-α was positive for pSTAT2 and, therefore, functioned in accordance to the classical JAK/STAT-pathway. By integrating ChIP-chip STAT2 and pSTAT2 data, surprisingly, several ISG promoters were occupied with unphosphorylated STAT2 either before or after IFN-α treatment or both. The expression of the genes Herc5, Pitx2, and Cav1 was affected when unphosphorylated STAT2 no longer bound to the promoter in response to IFN treatment. This finding demonstrates that nuclear unphosphorylated STAT2 can regulate positively or negatively ISG expression.

STAT2 not only promotes ISG-induction but can also suppress gene expression in response to IFNs. An example of gene repression by STAT2 is the p53 paralog DNp73 oncogene found upregulated in multiple human cancers (Testoni and others 2011a). IFN-α treatment causes substantial chromatin remodeling of the DNp73 promoter, which contains a functional ISRE site. Recruitment of STAT2 and the polycomb group protein Ezh2 to the promoter increased histone H3K27-methylation and transcriptional repression. These recent studies clearly reveal how much there is left to decipher about the molecular mechanisms of STAT2-dependent transcriptional regulation at the chromatin level.

STAT2 in Antiviral Immunity

The generation of STAT2 knockout (KO) mice has made it possible to study in greater detail the physiological role of STAT2 (Park and others 2000). STAT2 KO mice breed and develop normally, but compared with wild-type mice, they are vulnerable to infection when challenged by various viruses, among them vesicular stomatitis virus, dengue virus (Perry and others 2011), and sindbis virus (Gil and others 2001). To evade the antiviral protective effects of IFNs, certain viruses have developed strategies to impair the IFN signaling pathway by specifically targeting STAT2. Cells expressing dengue virus replicon show loss of human STAT2 protein (Jones and others 2005). Dengue virus protein NS5 was later identified as a vital component in reducing the levels of STAT2 protein by binding STAT2 and targeting it for degradation (Ashour and others 2009). However, this viral defense mechanism is specific for human and not mouse STAT2 (Ashour and others 2010). Human STAT2/NS5 interactions have been mapped to the STAT2 CC domain. A different study, however, contradicts this finding in that NS5 does not cause STAT2 degradation but instead hinders STAT2 tyrosine phosphorylation (Mazzon and others 2009).

Members of the Paramyxovirus family of viruses employ a different strategy to subvert the IFN antiviral response. STAT2 associates with the V protein of hendra, measles, and nipah viruses and becomes sequestered in the cytoplasm (Rodriguez and others 2002, 2003; Ramachandran and others 2008). Measles virus (MV) and lymphocytic choriomeningitis virus (LCMV) can also suppress the antiviral effects of IFN by employing a different tactic. Rather than reducing STAT2 levels, sequestrating STAT2 in the cytoplasm, or preventing its tyrosine phosphorylation, these viruses rely on IFN-activated STAT2 to suppress the immune system via inhibition of dendritic cell (DC) differentiation (Hahm and others 2005). The role of STAT2 in DC development was confirmed in STAT2 hypomorphic mutant mice, which showed suppressed antiviral responses (Chen and others 2009). In contrast, immunity against mouse cytomegalovirus (MCMV) relies on STAT2 activity independent of type I IFNs. Deletion of the antagonistic MCMV viral protein pM27 enhanced the tyrosine phosphorylation of STAT2 and antiviral responses induced by IFN-γ (Zimmermann and others 2005). Thus STAT2 remains critical in limiting viral replication and essential in innate antiviral immunity.

STAT2-Dependent STAT1-Independent Signaling Pathways

As a component of ISGF3 and other STAT2-containing signaling complexes, it is clear that STAT2 plays an essential role in the transcriptional responses to IFNs by either direct or indirect association with DNA and with a strong dependence on STAT1. However, evidence continues to accumulate in that type I IFN induction of ISGs and biological outcomes can occur in a STAT2-dependent, STAT1-independent manner. In an earlier study investigating STAT1-independent IFN-signaling, IFN-α and IFN-γ were found to regulate proliferative responses differently in cells of the mononuclear phagocyte lineage. IFN-α induced an antiproliferative effect, while IFN-γ promoted proliferation on myeloid precursor cells lacking STAT1 (Gil and others 2001). Although the involvement of STAT2 in the IFN response was not part of this study, it can be postulated that STAT2 signaling complexes lacking STAT1 may have been involved in promoting the antiproliferative effects of type I IFNs. In 2005 and 2006, 2 studies were published that uncovered type I IFN-mediated STAT2-dependent signaling pathways in the absence of STAT1. In the first study, MV infection of transgenic mice expressing MV receptor human signaling lymphocytic activation molecule on DC caused immunosuppression through type I IFNs interfering with bone-marrow DC development via STAT2-dependent STAT1-independent signaling (Hahm and others 2005). MV infection of bone-marrow cells increased STAT2 protein levels as well as tyrosine phosphorylation of STAT2. Similar findings were made with LCMV. MV and LCMV induced inhibition of DC expansion, and immunosuppression was the same in wild-type and STAT1 KO mice whereas STAT2 KO mice showed normal DC development. Therefore, the proposed molecular mechanism involves type I IFN induction by MV or LCMV followed by type I IFN/STAT2 signaling. In the second study, Sarkis and others (2006) studied the transcriptional regulation of the antiviral gene APOBEC3G (A3G; cytidine deaminase enzyme) by IFN-α in different cell types. Induction of A3G by IFN-α was detected in human liver cells and macrophages but not in the human T-cell line H9, thus indicating cell-type differences. Expression of A3G and other ISGs (PKR, ISG15, and Mx1) in Hep3B liver cells was drastically reduced when either STAT2 or IRF9 was knocked down. Surprisingly, expression of this subset of ISGs was not affected by STAT1 knockdown, revealing that IFN-α -induced STAT1 activation is not required but STAT2 activation is critical for the expression of a subset of ISGs in liver cells. In contrast, IFN-α and IFN-γ induction of ISGs was dependent on STAT1 when examined in 293T kidney cells, suggesting cell-type selectivity to the IFN transcriptional response. Recent studies continue to support the activation of IFN/STAT2 signaling pathways without dependence on STAT1. For instance, type I IFN induction of Adar1, an RNA-specific deaminase, was shown to be up-regulated via STAT2 activation in STAT1 KO mouse embryonic fibroblasts (George and others 2008). In a different report, Perry and others (2011) expanded on the earlier observations made by Gil and others (2001) in which mice lacking STAT1 were more resistant to sindbis virus and murine cytomegalovirus than IFNAR1- and IFNGR1-deficient mice. In this study, resistance to dengue virus (serotype 2 strain S221) infection and mortality among WT, STAT1KO, STAT2KO, and STAT1/STAT2 double KO mice were compared. WT and single KO mice remained resistant to virus infection. In contrast, the antiviral effect of IFN was abolished in STAT1/IFNAR1 double KO mice leaving STAT2 as the presumed signaling factor involved in the antiviral response observed in STAT1 KO mice. Gene expression analysis of 84 known ISGs showed a group of genes that were induced in the spleens isolated from STAT1 KO mice after dengue infection, of which a subset of them was not expressed in the spleens of STAT1/STAT2 double KO mice, indicating a requirement of STAT2, but not STAT1, in mediating induction of those genes. A similar pattern was reproduced in bone-marrow-derived macrophages (BMM) established from these animals and infected with dengue virus, which employs cells of monocyte/macrophage lineage for its replication. Some of the IFN-induced antiviral genes not expressed in STAT1/STAT2 double KO mice were the same genes mentioned earlier to be induced without dependence on STAT1 (ie, OAS1 and MX1) (Hahm and others 2005; Sarkis and others 2006). Further studies demonstrated that in STAT1 KO BMM, dengue infection performed in vitro did not interfere with the tyrosine phosphorylation and nuclear translocation of STAT2. ChIP assays confirmed that after dengue infection, STAT2 associated with the promoters of the OAS1 and IRF1 genes. What remains undetermined is whether IRF9 participates in the activation of STAT1-independent genes as a part of the STAT2:IRF9 complex or whether STAT2 binds DNA directly with additional unidentified proteins. Thus far, these data suggest that STAT1 and STAT2 can function independently in the regulation of a subset of ISGs.

STAT2 Regulation

STAT2 is ubiquitously expressed in most cell types, which most likely is an indicator of the importance of IFN signaling in the innate response against viral infections. STAT2 contains a weak ISRE in its promoter region that triggers up-regulation of STAT2 during type I IFN signaling (Yan and others 1995). The importance of an increase in STAT2 expression during type I IFN signaling has not been determined. However, STAT1 up-regulation during IFN signaling has proved to be important for prolonging the induction of ISGs, and a similar role for STAT2 up-regulation could be feasible (Cheon and Stark 2009).

STAT2 can be negatively regulated by the small G-protein Ras. Constitutively activated Ras, due to mutations in its N-terminal region, is often found in tumor cells and has in several studies been shown to inhibit the cellular response to type I IFN (Klampfer and others 2003; Battcock and others 2006; Noser and others 2007; Christian and others 2009). In the human colon carcinoma cell line HCT116, activated Ras suppressed STAT2 mRNA and protein expression, as well as STAT2-inducibility in response to IFN-γ (Klampfer and others 2003). Mouse NIH3T3 fibroblasts expressing a constitutively active Ras had compromised type I IFN signaling and anti-viral protection due to a decrease in STAT2 mRNA and protein expression (Battcock and others 2006; Christian and others 2009). Inhibition of MEK2, a mitogen-activated protein kinase functioning downstream of Ras, restored STAT2 protein expression and anti-viral efficiency of type I IFN (Christian and others 2009). The events downstream of MEK2 leading to suppression of STAT2 were not investigated and remains unknown.

Acetylation is a post-translational modification that affects chromatin remodeling and, consequently, gene expression. The consensus view on acetylation in IFN-α signaling has been that a general decrease in acetylation is beneficial for ISG induction (Nusinzon and Horvath 2003; Chang and others 2004). Tang and and others (2007) presented evidence that, in response to IFN-α, the histone acetylase CBP is recruited to IFNAR2 leading to subsequent acetylation of IFNAR2, IRF9, and STAT2 on multiple lysine (K) amino residues. Acetylation of K390 located in the DBD of STAT2 was suggested to destabilize the anti-parallel STAT2:STAT1 heterodimer that exists in untreated cells to facilitate the forming of the parallel STAT2:STAT1 heterodimer after tyrosine phosphorylation. Mutation of STAT2-K390 to arginine increased binding of STAT2 to STAT1 in untreated cells, and also disrupted induction of ISGs in response to IFN-α. So far, this is the only study that has reported on the importance of CBP-mediated STAT2 acetylation during ISGF3-induction.

STAT2 in the Antigrowth and Apoptotic Effects of IFNs

In addition to their antiviral effects, IFNs can also inhibit cell growth and even trigger an apoptotic response. STAT2, as a part of ISGF3, has been shown to play a critical role in these processes. STAT2 is important for the induction of apoptosis in human H123-cells, a Jurkat T-cell subline, as loss of STAT2 protected these cells from IFN-α-induced apoptosis (Romero-Weaver and others 2010). The exact mechanism of IFN-α-induced apoptosis is not fully clear, but in the case of H123-cells, apoptosis was promoted by a loss of mitochondrial membrane potential and caspase-3 activation. A possible involvement of STAT2 in the induction of apoptosis by other agents has not been addressed directly, but as observed by Du and others (2009), a Daudi subline lacking STAT2 was resistant not only to the apoptotic-inducing effects of type I IFNs but also to camptothecin, staurosporine, and doxorubicin, indicating that STAT2 is involved in apoptosis signaling outside its role in the JAK/STAT-pathway.

Although an apoptotic phenotype is sometimes observed in cells treated with type I IFNs, the majority of cells exposed to type I IFN respond with a decreased proliferation rate. The molecular mechanisms that determine how a cell chooses its fate have not been identified. One hypothesis is that the strength and duration of the signal triggered by IFN, in the form of ISGF3-mediated ISG-induction, may determine induction of apoptosis. This is supported by Scarzello and others (2007), where a STAT2-Y631F mutant showed prolonged STAT2 activation together with STAT1 in response to type I IFN, which coincided with increased cell death compared with cells expressing only wild type STAT2.

A link between STAT2 and tumorigenesis was observed in transgenic mice expressing constitutively IFN-α in the brain (Wang and others 2003). Under these conditions, STAT2 KO mice developed tumors in the brain. The production of IFN-γ and STAT1-dependent expression of the sonic hedgehog gene, implicated in the genesis of medulloblastoma (that was not detected in STAT1KO), was presumed to have led to the formation of tumors. Therefore, a loss of STAT2 in the continuous presence of type I IFN has adverse effects. Interestingly, mouse cells devoid of STAT2 have been shown to respond to IFN-α in an IFN-γ like manner by inducing the expression of an IFN-γ regulated gene that is not induced by IFN-α in wild-type cells (Zhao and others 2007). In a different study, STAT2KO mice were subjected to chemical-induced carcinogenesis and found to develop fewer tumors when compared with wild-type mice (Romero-Weaver and others 2010). Whether the protective effect of STAT2 deficiency is dependent on IFN signaling has yet to be determined.

Concluding Remarks

It is well recognized that STAT2 is an essential component in the transcriptional responses to IFNs. Several studies confirm that a deficiency in STAT2 has detrimental effects to the host, as this affects the biological outcomes of IFNs. As reviewed here, our initial view of how STAT2 regulates IFN induced gene transcription is changing. Not all the effects of STAT2 are dependent on STAT1. STAT2 can form heterodimers with other STATs and associate with additional transcription factors to promote ISG transcription. A most surprising discovery is that unphosphorylated STAT2 binds a subset of ISG promoters. Release of unphosphorylated STAT2 from the chromatin in response to IFN stimulation can either suppress or induce gene transcription. The mechanism of how unphosphorylated STAT2 regulates gene transcription remains to be defined. While significant progress has been made in understanding the biological role of STAT2, many aspects of STAT2 remain to be elucidated. For instance, it is unclear how STAT2 transcriptional activity is switched off. The identity of the protein tyrosine phosphatase that selectively dephosphorylates STAT2 remains unknown. Are there additional post-translational modifications occurring in STAT2 in response to IFN treatment, such as serine phosphorylation and lysine acetylation, that selectively modulate transcriptional responses to IFNs? Moreover, it has yet to be determined whether single-nucleotide polymorphisms (SNPs) in STAT2 influence IFN signaling. This information is critical, because STAT2 SNPs may influence the therapeutic effects IFNs, which are currently used in the treatment of a variety of diseases. Future studies are also expected to elucidate the precise role of STAT2 in tumorigenesis and whether the presence or absence of STAT2 may be implicated in tumor development.

Acknowledgments

The authors thank Chanyu Yue and Kevin Kotredes for their critical reading of this article.

Author Disclosure Statement

There are no competing financial interests in connection with this article to be reported.

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