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. 1987 Dec;7(12):4194–4203. doi: 10.1128/mcb.7.12.4194

Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.

C L Peterson 1, K L Calame 1
PMCID: PMC368100  PMID: 3125419

Abstract

We have begun to purify and characterize several proteins which bind to the mouse immunoglobulin heavy-chain enhancer to understand the molecular interactions important for enhancer activity. Three proteins which bind to different sites on the immunoglobulin heavy-chain enhancer have been chromatographically separated and partially purified. One protein binds a site which has not been reported previously and does not bind to other reported protein-binding sites on the immunoglobulin heavy-chain enhancer. Binding-site boundaries for the three partially purified proteins have been precisely mapped by methylation interference, DNase I footprinting, and orthophenanthroline/copper chemical nuclease footprinting. We have also characterized these three proteins with respect to dissociation rate constants.

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Selected References

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