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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Hepatology. 2013 May 14;58(1):284–292. doi: 10.1002/hep.26333

Figure 1.

Figure 1

TLC induces plasma membrane translocation of PKCε and retrieval of MRP2. HuH-NTCP cells were incubated with 10 μM TLC, 1μM PMA or 100μM CPT-cAMP for 15 followed by biotinylation of cell surface proteins and immunoblot analysis of biotinylated PKCε (PM-PKCε, 88kD) and E-cadherin (E-Cad as loading control, 135kD). For MRP2 assay, cells were treated with 10μM TLC (25 min) or 100μM CPT-cAMP (15 min) followed by and immunoblot analysis of biotinylated MRP2 (PM-MRP2, 195kD) and E-cadherin. Typical PM-MRP2, PM-PKCε and E-Cad immunoblots are shown in the upper panel and results of densitometric analysis (Mean±SEM, n=5) are shown in the bar graph. *Significantly different (p<0.05) from respective control (CON) values.