TLC-induced MARCKS phosphorylation is mediated via PKCε. HuH-NTCP cell transfected with HA-tagged DN-PKCε were treated with 10μM TLC, 1μM PMA or 100μM CPT-cAMP for 15 followed by immunoblot analysis of HA, PKCε, pMARCKS and actin. Typical immunoblots are shown in the upper panel and results of densitometric analysis (Mean±SEM, n=5) are shown in the bar graph. *Significantly different from empty (EV) control (CON), &significantly different from DN-PKCε control and #significantly different from respective empty vector (EV) values.