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. 1994 Mar;1(2):250–252. doi: 10.1128/cdli.1.2.250-252.1994

Salmonella-specific monoclonal antibodies against recombinant Salmonella typhi 36-kilodalton porin.

V Kissel 1, C Gonzalez 1, M Astudillo 1, A Godard 1, B Wachman 1, F C Cabello 1
PMCID: PMC368239  PMID: 7496957

Abstract

Mouse monoclonal antibodies were raised against recombinant Salmonella typhi 36-kDa porin monomer. Specificities of 16 monoclonal antibodies were analyzed as reactivity patterns in dot immunobinding and Western blot (immunoblot) assays using isolated outer membrane proteins of gram-negative bacteria and cloned purified S. typhi porin monomers and trimers. Four monoclonal antibodies were specific for Salmonella spp.

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Selected References

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  1. Agüero J., Mora G., Mroczenski-Wildey M. J., Fernandez-Beros M. E., Aron L., Cabello F. C. Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli. Microb Pathog. 1987 Dec;3(6):399–407. doi: 10.1016/0882-4010(87)90010-6. [DOI] [PubMed] [Google Scholar]
  2. Appassakij H., Bunchuin N., Sarasombath S., Rungpitarangsi B., Manatsathit S., Komolpit P., Sukosol T. Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen. J Clin Microbiol. 1987 Feb;25(2):273–277. doi: 10.1128/jcm.25.2.273-277.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aron L., Di Fabio J., Cabello F. C. Salmonella typhi O:9,12 polysaccharide-protein conjugates: characterization and immunoreactivity with pooled and individual normal human sera, sera from patients with paratyphoid A and B and typhoid fever, and animal sera. J Clin Microbiol. 1993 Apr;31(4):975–978. doi: 10.1128/jcm.31.4.975-978.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Aron L., Faundez G., Gonzalez C., Roessler E., Cabello F. Lipopolysaccharide-independent radioimmunoprecipitation and identification of structural and in vivo induced immunogenic surface proteins of Salmonella typhi in typhoid fever. Vaccine. 1993;11(1):10–17. doi: 10.1016/0264-410x(93)90334-t. [DOI] [PubMed] [Google Scholar]
  5. Benz R. Structure and function of porins from gram-negative bacteria. Annu Rev Microbiol. 1988;42:359–393. doi: 10.1146/annurev.mi.42.100188.002043. [DOI] [PubMed] [Google Scholar]
  6. Calderón I., Lobos S. R., Rojas H. A., Palomino C., Rodríguez L. H., Mora G. C. Antibodies to porin antigens of Salmonella typhi induced during typhoid infection in humans. Infect Immun. 1986 Apr;52(1):209–212. doi: 10.1128/iai.52.1.209-212.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chatfield S. N., Dorman C. J., Hayward C., Dougan G. Role of ompR-dependent genes in Salmonella typhimurium virulence: mutants deficient in both ompC and ompF are attenuated in vivo. Infect Immun. 1991 Jan;59(1):449–452. doi: 10.1128/iai.59.1.449-452.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Faundez G., Aron L., Cabello F. C. Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains. J Clin Microbiol. 1990 May;28(5):894–897. doi: 10.1128/jcm.28.5.894-897.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gabay J., Schwartz M. Monoclonal antibody as a probe for structure and function of an Escherichia coli outer membrane protein. J Biol Chem. 1982 Jun 25;257(12):6627–6630. [PubMed] [Google Scholar]
  10. Hofstra H., Dankert J. Major outer membrane proteins: common antigens in enterobacteriaceae species. J Gen Microbiol. 1980 Jul;119(1):123–131. doi: 10.1099/00221287-119-1-123. [DOI] [PubMed] [Google Scholar]
  11. Kuusi N., Nurminen M., Saxen H., Valtonen M., Mäkelä P. H. Immunization with major outer membrane proteins in experimental salmonellosis of mice. Infect Immun. 1979 Sep;25(3):857–862. doi: 10.1128/iai.25.3.857-862.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mizuno T., Chou M. Y., Inouye M. A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene. J Biol Chem. 1983 Jun 10;258(11):6932–6940. [PubMed] [Google Scholar]
  13. Mroczenski-Wildey M. J., Di Fabio J. L., Cabello F. C. Invasion and lysis of HeLa cell monolayers by Salmonella typhi: the role of lipopolysaccharide. Microb Pathog. 1989 Feb;6(2):143–152. doi: 10.1016/0882-4010(89)90017-x. [DOI] [PubMed] [Google Scholar]
  14. Muthukkumar S., Muthukkaruppan V. R. Detection of porin antigen in serum for early diagnosis of mouse infections with Salmonella typhimurium. FEMS Microbiol Immunol. 1992 Feb;4(3):147–153. doi: 10.1111/j.1574-6968.1992.tb04981.x. [DOI] [PubMed] [Google Scholar]
  15. Schnaitman C. A. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol. 1971 Oct;108(1):545–552. doi: 10.1128/jb.108.1.545-552.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Singh S. P., Upshaw Y., Abdullah T., Singh S. R., Klebba P. E. Structural relatedness of enteric bacterial porins assessed with monoclonal antibodies to Salmonella typhimurium OmpD and OmpC. J Bacteriol. 1992 Mar;174(6):1965–1973. doi: 10.1128/jb.174.6.1965-1973.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spierings G., Elders R., van Lith B., Hofstra H., Tommassen J. Characterization of the Salmonella typhimurium phoE gene and development of Salmonella-specific DNA probes. Gene. 1992 Dec 1;122(1):45–52. doi: 10.1016/0378-1119(92)90030-s. [DOI] [PubMed] [Google Scholar]
  18. Torensma R., Visser M. J., Aarsman C. J., Poppelier M. J., van Beurden R., Fluit A. C., Verhoef J. Monoclonal antibodies that detect live salmonellae. Appl Environ Microbiol. 1992 Dec;58(12):3868–3872. doi: 10.1128/aem.58.12.3868-3872.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tufano M. A., Ianniello R., Galdiero M., De Martino L., Galdiero F. Effect of Salmonella typhimurium porins on biological activities of human polymorphonuclear leukocytes. Microb Pathog. 1989 Nov;7(5):337–346. doi: 10.1016/0882-4010(89)90037-5. [DOI] [PubMed] [Google Scholar]
  20. Udhayakumar V., Muthukkaruppan V. R. Protective immunity induced by outer membrane proteins of Salmonella typhimurium in mice. Infect Immun. 1987 Mar;55(3):816–821. doi: 10.1128/iai.55.3.816-821.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Poucke L. S. Salmonella-TEK, a rapid screening method for Salmonella species in food. Appl Environ Microbiol. 1990 Apr;56(4):924–927. doi: 10.1128/aem.56.4.924-927.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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