FIGURE 7.
uORF peptides inhibit EPHX1 protein translation in wheat-germ extract. (A,C) Wheat-germ extract was programmed with full-length EPHX1 E1-b plasmid construct and in vitro transcription/translation occurred in the presence of [35S]-methionine. Different concentrations of uORF peptides and scrambled peptides were titrated into the wheat-germ extract system. (B,D) Quantification of EPHX1 E1-b protein expression in the presence of varying peptide concentrations, relative to control (no peptide). The data shown depict means and S.D. values from two separate experiments. (Varying peptide concentrations vs. no peptide; [*] P < 0.05; [**] P < 0.01; [***] P < 0.001; Student’s t-test.)