Skip to main content
NIHPA Author Manuscripts logoLink to NIHPA Author Manuscripts
. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cancer Epidemiol. 2013 Mar 26;37(4):424–427. doi: 10.1016/j.canep.2013.03.003

Germline HOXB13 p.Gly84Glu Mutation and Risk of Colorectal Cancer

Mohammad R Akbari a,b, Laura N Anderson c, Daniel D Buchanan d, Mark Clendenning d, Mark A Jenkins e, Aung Ko Win e, John L Hopper e, Graham G Giles f, Robert Nam g, Steven Narod a,b, Steven Gallinger c,h, Sean P Cleary c,h
PMCID: PMC3684005  NIHMSID: NIHMS464456  PMID: 23541221

Abstract

Introduction

The HOXB13 p.Gly84Glu mutation has recently been associated with an increased risk of prostate cancer but the association of other cancer sites with this allele has not been assessed. Data has suggested that HOXB13 expression levels are decreased in colorectal cancer (CRC) cell lines indicating this gene may be involved in colorectal tumourigenesis.

Methods

To evaluate a potential association of this mutation with CRC, we genotyped the mutation in 2,695 CRC cases and 4,593 controls from population-based registries in Canada and Australia.

Results

The HOXB13 p.Gly84Glu mutation was more common in CRC cases than controls (0.48% vs. 0.17%, p=0.02) indicating a significant association between the HOXB13 variant and CRC risk (OR = 2.8; 95%CI: 1.2-6.8). This association was attenuated but remained significant with the inclusion of previously published and publicly available genotype data. Pedigree analysis of cases and controls revealed that 7/21 HOXB13 mutation carriers had a family history of prostate cancer.

Discussion

This report is the first to suggest a risk of CRC associated with mutations in the HOXB13 gene. These findings require further validation but may be of importance in the screening and genetic counseling of families known to carry the HOXB13 p.Gly84Glu mutation.

Keywords: Colorectal cancer, risk, HOXB13 gene, genetic polymorphism

Introduction

Up to one third of colorectal cancer (CRC) cases have a family history of the disease suggesting a heritable component in the cases. Mutations in APC, MUTYH and mismatch repair genes account for only a minority of familial cases, while results from recent genome-wide association studies have identified multiple low-penetrance variants that when combined explain a proportion of the heritability of CRC (1). Despite this relatively advanced understanding of CRC genetics, the etiology of the vast majority of familial cases remains unexplained. Recently, a novel germline mutation, p.Gly84Glu (rs138213197), in exon one of the HOXB13 gene was shown to increase risk of prostate cancer by 5-10 fold (2, 3). The risk was higher in association with familial cases. HOXB13 is a transcription factor gene that belongs to the HOXB gene cluster at chromosome 17 (4), is involved in embryonic development of different organs including the digestive tract (5) and regulates transcription of androgen receptor (AR) target genes (6). Previous studies suggest that HOXB13 might be involved in colorectal tumorigenesis. HOXB13 expression levels are decreased in colon tumour cells compared to normal cells (7), and hypermethylation of a CpG island upstream of HOXB13 was reported as a potential mechanism for down regulation of HOXB13 in CRC (8). Based on these data, we hypothesized that the recently described HOXB13 p.Gly84Glu mutation may be associated with CRC risk.

Materials and Methods

We genotyped the HOXB13 p.Gly84Glu mutation in germline DNA of 2,695 population-based CRC cases and in 4,593 controls. Subjects included 1,952 CRC cases and 1,197 controls from the Ontario Familial Colon Cancer Family Registry (OFCCR) and 743 CRC cases and 246 controls from Australasian Colorectal Cancer Family Registry (ACCFR). The OFCCR and ACCFR are two population-based sites of the National Cancer Institute supported Colorectal Cancer Family Registries consortium. The details of this consortium including recruitment strategies have been previously published (9). Briefly, the OFCCR recruited incident CRC cases (1997-2002) from the population-based Ontario Cancer Registry. Cases were stratified into high risk (Amsterdam criteria) (n=106), intermediate risk (n=920) and low risk (n=926) according to family histories, age of onset and pathologic characteristics; all high and intermediate risk cases and a 25% random sample of low-risk cases were subsequently recruited. For the Ontario cases, controls with no prior personal history of cancer were recruited through residential telephone lists as well as the Ontario Ministry of Finance property-assessment file for the year 2000 (10). The ACCFR recruited CRC cases aged 18-59 from the Victoria Cancer Registry and controls were identified through local electoral roles. Those cases (n=106) that met Amsterdam Criteria were screened for mutations on mismatch repair genes and 23 cases were identified to have a pathogenic mutation in one of the mismatch repair genes. In both registries, Cases of confirmed or suspected familial adenomatous polyposis were excluded and control subjects were age- and sex-frequency matched to CRC cases. The population frequency of the HOXB13 p.Gly84Glu variant is reported to be very low (<1%) in previous studies and therefore we sought to enlarge our control group to generate a precise estimate. In addition, 925 female controls were obtained from the Health Watch (HW) program at Womens College hospital. These are healthy women with no prior history of cancer who had attended a multimodal screening clinic for well women at Women’s College Hospital in Toronto (but not for colorectal screening); and 2,225 male controls from a case-control study in Toronto that have been previously genotyped (3). These men had an elevated PSA but had a negative prostate biopsy. The study was approved by the ethics review board at all participating institutions.

In a second phase, we also used the allele frequency of the mutation reported among 6,481 study subjects of the NHLBI (National Heat, lung and blood Institute) Exome Sequencing Project (ESP6500) (11) and 1,128 individuals from the 1000 Genomes Project (G1000, release7) (12).

Genotyping of the HOXB13 p.Gly84Glu mutation among cases and controls was performed using a combination of TaqMan assay on ABI 7500 fast and ViiA7 real-time systems (Applied Biosystems Co., Foster City, CA, USA) and iPLEX chemistry on a MALDI-TOF MassARRAY system (Sequenom Inc., San Diego, CA, USA). All mutation carriers identified by genotyping were confirmed by direct sequencing using the BigDye Terminator Cycle Sequencing kit on an ABI 3500XL DNA Analyzer (Applied Biosystems Co., Foster City, CA, USA). We compared the frequency of the HOXB13p.Gly84Glu mutation between cases and controls using Fisher’s exact test and calculated odds ratios (OR) and their 95% confidence intervals (CI) based on 2×2 table analysis of cases and controls.All statistical tests were two-sided and p values <0.05 were considered statistically significant. Because of the very small number of carriers of the variant alleles in the control population, we combined the control groups to provide a composite estimate of prevalence and it was not practical to perform subset analyses. Adjustment for ethnicity, age and gender using a multivariable logistic regression model was not possible given the low number of positive observations of the mutation.

Results

The characteristics of cases and controls are shown in Table 1. Thirteen heterozygote carriers of the HOXB13 p.Gly84Glu mutation were identified among 2,695 cases (0.48%) and 8 carriers were seen among 4,593 controls (0.17%) (OR = 2.8, 95% CI = 1.2 - 6.7, P = 0.02). Several carriers among the cases and controls had a family history of CRC and/or prostate cancer (See Table 2). Among the 13 CRC mutation carriers, 5 had a family history of CRC and 4 had a family history of prostate cancer. Of the 8 control subjects with HOXB13 mutation for whom we had family history information (OFCCR, ACCFR, HW and Prostate study controls), 3 had a family history of CRC and 3 had a family history of prostate cancer. When the analysis was limited to 1,395 cases who had a family history of CRC or prostate cancer, the odds ratio was 3.3 (0.57% vs. 0.17%, 95%CI: 1.2-8.8, P = 0.01). Based on prior testing, none of the HOXB13 mutation carriers harboured mutations in MUTYH and none of the Ontario cases tested had truncating mutations in APC, although cases of polyposis were excluded from both studies. Two HOXB13 mutation carriers were found to have mutations in MLH1 and MSH6, respectively, and their tumors demonstrated high microsatellite instability.

Table 1.

Characteristics of the case and control subjects

Variable Cases Controls p-value
OFFCR ACCFR OFFCR ACCFR Health
Watch
Prostate
Study
Total Number, n 1,952 743 1,197 246 925 2,225
Agea, Mean (range) 56.8
(15-90)
47.0
(18-59
63.6
(29-79)
48.5
(20-61)
56.0 (27-
95)
70.9 (44-
97)
<0.001
Gender, n
(%)
Male 884
(45.3)
389
(52.3)
622
(52.0)
130
(52.8)
0 (0.0) 2,225
(100)
<0.001
Female 1,068
(54.7)
354
(47.7)
575
(48.0)
116
(47.2)
925
(100)
0 (0.0)
Ethnicity, n
(%)
White 1,815
(93.0)
704
(94.7)
1,126
(94.1)
236
(95.9)
884
(95.6)
1,787
(80.3)
<0.001
Otherb 137
(7.0)
39 (5.3) 71 (5.9) 10 (4.1) 41 (4.4) 438 (19.7)
HOXB13 p.Gly84Glu
carriers, n (%)
11 (0.6) 2 (0.2) 4 (0.3) 1 (0.4) 1 (0.1) 2 (0.1) 0.02
a

Age for cases is their age of diagnosis and for controls is their age of consent

b

Including south and east Asians, south Americans and Africans.

Table 2.

Clinical characteristics of 21individuals carrying germline HOXB13G84E mutation

No. Studya Subjects Gender Ageb at diagnosis, y Family historyc
1 OFCCR Case M 73 None
2 OFCCR Case M 63 None
3 OFCCR Case F 66 None
4 OFCCR Case M 51 Father, 5 Paternal Uncles
(PrCa, 74, 60, 63, 72, 74, 74)),
Maternal Grandfather (CRC,
81)
5 OFCCR Case F 59 Paternal Uncle, Maternal
Grandfather (PrCa, 78, 80)
6 OFCCR Case M 45 2 Maternal Uncles (CRC, 69,
75), 2 Paternal Uncles
(PrCa,UK, UK)
7 OFCCR Case F 59 None
8 OFCCR Case M 42 Paternal Uncle (CRC, 60)
9 OFCCR Case F 69 Maternal Uncle, Maternal
Grandfather (CRC, UK, UK)
10 OFCCR Case M 39 None
11d OFCCR Case M 40 Father, Mother, Sister,
Maternal Uncle (CRC, 58, 54,
56, 54)
12e ACCFR Case M 22 None
13 ACCFR Case F 42 Father, Brother (PrCa, 64, 49)
14 ACCFR Control M 52 Father, Paternal Uncle,
Maternal Uncle (CRC, 44, UK,
UK)
15 OFCCR Control M 59 Father (PrCa)
16 OFCCR Control F 73 None
17 OFCCR Control M 68 None
18 OFCCR Control F 54 Father, Mother, Maternal
Grandfather (CRC), Maternal
Uncle (PrCa)
19 HW Control F 57 Brother (CRC, 54)
20 PC Control M 59 Father, Uncle (PrCa, 79, 85)
21 PC Control M 66 None
a

Ontario Colon Cancer Family Registry (OFCCR), Australasian Colorectal Cancer Family Registry (ACCFR), Health Watch Study (HW), Prostate Cancer Study (PC)

b

Age for cases is their age of diagnosis and for controls is their age of consent

c

Family history of colorectal cancer (CRC) and prostate cancer (PrCa) among the first and second degree relatives of the probands. The age at diagnosis was provided for whom it was available.UK= age of diagnosis is unkown

d

This individual carried a MLH1 mutation (c.1217_23dupGTCAGCC, p.Q409fsX85)

e

This individual carried a MSH6 mutation (c.3311_3312delTT, p.Phe1104TrpfsX3)

Given the small number of controls with the mutation (n = 8) we sought to incorporate mutation frequency data from publicly available sources. The minor allele frequency (MAF) of HOXB13 p.Gly84Glu among the 1,128 individuals with available exome sequencing data in release 7 of the G1000 project is 0.001, equal to 2 mutated alleles. Twenty mutated alleles were also reported among the 6,481 individuals of ESP6500 project. Since all carriers in these two studies were heterozygous, there are 22 carriers in total among the 7,609 individuals in G1000 and ESP6500 projects combined (0.29%). Including these controls with our own set of controls and comparing the mutation frequency with that in CRC cases shows that HOXB13 p.Gly84Glu is associated with increased risk of colon cancer, but with a lower odds ratio (OR = 2.0, 95%CI = 1.0 to 3.8, P = 0.04). Combining the data presented by Alanee et al. (13) with the case-control data from our series yields an odds ratio of 2.6 (95%CI 1.12-6.01, p=0.03)

Discussion

We are the first to report a significant association between the HOXB13 p.Gly84Glu mutation and CRC risk. We identified a carrier frequency of 0.48% among CRC cases from two distinct population-based CRC registries. Many of the carriers identified had a family history of colon and/or prostate cancer further suggesting that this variant may prediscpose to both colorectal and prostate cancer. The carrier frequency among controls was 0.17% among subjects tested and 0.29% among subjects in publicly available databases. The carrier frequency in all controls combined in our series (0.17%) is similar to that of 0.1% and 0.2% seen in controls genotyped by Ewing et al. (2) and the 0.21% reported by Breyer et al (14). Alanee et al (13) reported a 0.1% carrier rate among 1,052 CRC cases and 1,650 controls; while this report did not suggest an increased risk of CRC; combining the data from Alanee et al (13) and this series still produces a significant association between HOXB13 and CRC risk. Given the rarity of this variant, our findings require further validation in other series of CRC cases and controls.

Two of the HOXB13 p.Gly84Glu carriers in our study also carried a mutation on one of the mismatch repair genes. This is not expected to confound the association of HOXB13 with colon cancer, since none of the mismatch repair genes are located in a same chromosome with HOXB13 and their inheritance is totally independent from HOXB13. However, observing the concurrent mutations on HOXB13 and mismatch repair genes among our study subjects raises a question about synergistic effect between carrying mutations on these two sets of genes together that could not be answered with small number of carriers we observed.

Since genes harbouring risk alleles can be expressed in multiple tissues, it is not surprising that some mutations may predispose to multiple different cancers. Mutations in DNA mismatch repair genes are associated with colorectal, endometrial and ureteric cancers; while APC mutations are associated with colorectal and multiple extracolonic neoplasms. Due to its low allele frequency (0.14%), this HOXB13 mutation accounts for only a small fraction of familial CRC cases. However, the effect size (OR: 2.0-2.8) of the HOXB13 p.Gly84Glu mutation that we have shown to be associated with CRC risk is similar or greater compared to that of other known CRC risk alleles (e.g. APC I1307K) or those identified via genome-wide association studies (15). In addition, since the recruitment of cases to the OFCCR favoured subjects with a family history of CRC, it is possible that this may lead to an overestimation of the population-based risk for this variant. The increased recruitment of familial cases may be responsible for the increased mutation frequency in these cases and enhanced our ability to detect a risk associated with this mutation. None the less, our finding of an association between HOXB13 mutations and CRC risk may lead to future investigations of the role of androgens and AR response genes in CRC and, if confirmed, will have implications for counseling families found to be carrying this mutation.

Acknowledgments

We acknowledge Dr. Kathleen A. Cooney from University of Michigan Medical school for providing the TaqMan assay design for genotyping HOXB13p.Gly84Glu.

This work was supported by the National Cancer Institute, National Institutes of Health under RFA # CA-95-011, the Ontario Registry for Studies of Familial Colorectal Cancer (U01 CA074783), the Australasian Colorectal Cancer Family Registry (U01 CA097735), and through cooperative agreements with members of the Colon Cancer Family Registry (CFRs) and P.I.s. The content of this manuscript does not necessarily reflect the views or policies of the National Cancer Institute or any of the collaborating centers in the CFRs, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government or the CFR.

Abbreviations

CRC

colorectal cancer

OFCCR

Ontario Familial Colon Cancer Family Registry

ACCFR

Australasian Colorectal Cancer Family Registry

HW

Health Watch Study

OR

odds ratio

CI

confidence interval

Footnotes

Conflict of Interest The authors have no conflicts of interest to declare.

Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

References

  • 1.Dunlop MG, Tenesa A, Farrington S, et al. Cumulative impact of common genetic variants and other risk factors on colorectal cancer risk in 42 103 individuals. Gut. 2012 doi: 10.1136/gutjnl-2011-300537. epub. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Ewing CM, Ray AM, Lange EM, et al. Germline mutations in HOXB13 and prostate-cancer risk. N Engl J Med. 2012;366(2):141–149. doi: 10.1056/NEJMoa1110000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Akbari MR, Trachtenberg J, Lee J, et al. Association between germline HOXB13 G84E mutation and risk of prostate cancer. J Natl Cancer Inst. 2012;104(16):1260–2. doi: 10.1093/jnci/djs288. [DOI] [PubMed] [Google Scholar]
  • 4.Zeltser L, Desplan C, Heintz N. Hoxb-13: a new Hox gene in a distant region of the HOXB cluster maintains colinearity. Development. 1996 Aug;122(8):2475–84. doi: 10.1242/dev.122.8.2475. [DOI] [PubMed] [Google Scholar]
  • 5.Kawazoe Y, Sekimoto T, Araki M, Takagi K, Araki K, Yamamura K. Region-specific gastrointestinal Hox code during murine embryonal gut development. Dev Growth Differ. 2002 Feb;44(1):77–84. doi: 10.1046/j.1440-169x.2002.00623.x. [DOI] [PubMed] [Google Scholar]
  • 6.Norris JD, Chang CY, Wittmann BM, et al. The homeodomain protein HOXB13 regulates the cellular response toandrogens. Mol Cell. 2009;36(3):405–416. doi: 10.1016/j.molcel.2009.10.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Jung C, Kim RS, Zhang H, et al. HOXB13 is downregulated in colorectal cancer to confer TCF4-mediated transactivation. Br J Cancer. 2005 Jun 20;92(12):2233–9. doi: 10.1038/sj.bjc.6602631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Ghoshal K, Motiwala T, Claus R, Yan P, Kutay H, Datta J, Majumder S, Bai S, Majumder A, Huang T, Plass C, Jacob ST. HOXB13, a target of DNMT3B, is methylated at an upstream CpG island, and functions as a tumor suppressor in primary colorectal tumors. PLoS One. 2010 Apr 29;5(4):e10338. doi: 10.1371/journal.pone.0010338. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  • 9.Newcomb PA, Baron J, Cotterchio M, et al. Colon Cancer Family Registry: an international resource for studies of the genetic epidemiology of colon cancer. Cancer Epidemiol Biomarkers Prev. 2007 Nov;16(11):2331–43. doi: 10.1158/1055-9965.EPI-07-0648. [DOI] [PubMed] [Google Scholar]
  • 10.Cotterchio M, McKeown-Eyssen G, Sutherland H, et al. Ontario familial colon cancer registry: methods and first-year response rates. Chronic Dis Can. 2000;21(2):81–6. [PubMed] [Google Scholar]
  • 11.NHLBI Exome Sequencing Project (ESP) Seattle, WA: Jul, 2012. Exome Variant Server. URL: http://evs.gs.washington.edu/EVS/ [Google Scholar]
  • 12.1000 Genomes Project (G1000) Seattle, WA: Jul, 2012. The 1000 Genomes Browser. URL: http://browser.1000genomes.org. [Google Scholar]
  • 13.Alanee S, Couch F, Offit K. Association of a HOXB13 variant and breast cancer. N Engl J Med. 2012;367:480–481. doi: 10.1056/NEJMc1205138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Breyer JP, Avritt TG, McReynolds KM, Dupont WD, Smith JR. Confirmation of the HOXB13 G84E Germline Mutation in Familial Prostate Cancer. Cancer Epidemiol Biomarkers Prev. 2012;21(8):1348–1353. doi: 10.1158/1055-9965.EPI-12-0495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Zanke BW, Greenwood CM, Rangrej J, et al. Genome-wide association scan identifies a colorectal cancer susceptibility locus on chromosome 8q24. Nat Genet. 2007 Aug;39(8):989–94. doi: 10.1038/ng2089. [DOI] [PubMed] [Google Scholar]

RESOURCES