Figure 4.

N. maritimus FtsZ-GFP (Nmar_1262) does not form filament-like structures in yeast. (a) Sequence alignment of the Nmar_1262 with the E. coli FtsZ (EcFtsZ). Regions highlighted in yellow and green in the EcFtsZ sequence are two conserved motifs for tubulin/FtsZ, GGGTGTG, and NVDFAD (T7-loop) for GTP binding and hydrolysis, respectively. Sequence alignment was performed using ClustalW2 program available from the website http://www.ebi.ac.uk/Tools/msa/clustalw2/. (b) Images of yeast cells expressing Nmar_1262-GFP and Nmar_1262**-GFP, in which the presumptive GTP-binding motif AGKAGSA was replaced with the conventional GGGTGTG. Scale bar: 10 μm.