Skip to main content
. 2013 Mar 19;2(3):383–401. doi: 10.1002/mbo3.84

Figure 6.

Figure 6

Localization of the PGN_2005 protein in Porphyromonas gingivalis. The cell lysates of the wild type and the PGN_2005 mutant were subjected to immunodetection with anti-PGN_2005 (A). Cell fractionation analysis from the wild type. W, C/P, and TM indicate the whole cell lysate, cytoplasm/periplasm, and total membrane fraction, respectively (B). Five micrograms of protein from the inner membrane (IM) or outer membrane (OM) fractions that were separated by sucrose density gradient centrifugation from the membrane fraction of the wild type were subjected to immunodetection with anti-PGN_2005, anti-HBP35, anti-Rgp, mAb 1B5, and mAb TDC-5-2-1 (C).