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. 2013 Mar 19;2(3):383–401. doi: 10.1002/mbo3.84

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© 2013 Published by John Wiley & Sons Ltd.

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Analysis of LPS to assess Wzz activity by heterologous complementation. Silver-stained polyacrylamide gel displaying the O-antigen LPS profiles of Escherichia coli W3110 (lane 1), EVV16 (wzzB) containing pMF19 (lane 2), EVV16/pMF19 containing pBAD vector control (lane 3), EVV16/pMF19 containing PGN_2005-expressing plasmid from Porphyromonas gingivalis (lane 4), EVV16/pMF19 containing WzzB-expressing plasmid (pWzzB-SF) from Shigella flexneri (lane 5), and EVV16/pMF19 containing WzzB-expressing plasmid (pWzzB-ST) from Salmonella typhimurium (lane 6) (A). Immunoblot analysis of the cell lysates was performed with anti-PGN_2005 mouse polyclonal antiserum to confirm the expression of the PGN_2005 protein in the E. coli EVV16 (pMF19) strain (B).