FIG 4.
JCPyV pseudoviruses with PML-associated mutations are not infectious. The cell types shown were plated in a 96-well plate O/N. Medium was aspirated, cells were infected with 1 × 107 particles/ml of wild-type (Mad-1) or mutant pseudovirus in incomplete medium without phenol red at 37°C for 1 h, then complete phenol red-free medium was added, and cells were incubated at 37°C for 72 h. Supernatant from infected cells was collected and analyzed for secreted luciferase using a luminometer. The average relative luciferase units for quadruplicate samples are shown in log scale. Error bars represent standard deviations. These data are representative of 3 experiments performed in triplicate.