Table 3.
Histological analysis of distal bone femur in Bdnf2lox/2lox/93 mice
| 6 months old |
||||
|---|---|---|---|---|
| Female |
Male |
|||
| Wild type | KO | Wild type | KO | |
| Osteoblast perim (ObS) (mm) | 0.5 ± 0.12 | 1.26 ± 0.14a | 0.72 ± 0.11 | 0.88 ± 0.08 |
| ObS/BS | 23.0 ± 1.3 | 22 ± 2.27 | 22.6 ± 2.27 | 16.6 ± 1.52 |
| N.Ob/BS | 14.5 ± 0.45 | 15.2 ± 1.67 | 15.3 ± 1.25 | 12.06 ± 1.41 |
| N.Oc/BS | 7.17 ± 1.03 | 3.17 ± 0.34a | 6.38 ± 0.69 | 4.15 ± 0.5 |
| N.Oc | 12.6 ± 1.39 | 18.4 ± 2.17 | 18.5 ± 2.34 | 25.2 ± 6.49 |
| Cortical thickness (μm) | 152 ± 25 | 246 ± 30a | 140 ± 16.5 | 243 ± 50a |
| Growth plate thickness (μm) | 100.5 ± 10.4 | 115.2 ± 10.9a | 82.7 ± 2.6 | 91.8 ± 10.1a |
Histomorphometric parameters were measured in equivalent regions of interest using sections of distal femurs from wild-type and 6-month-old Bdnf2lox/2lox/93 mice (n = 4–12 sections). Oc/BS (osteoclast number) was significant only in females and only when normalized per bone slice. ObS, Osteoblast surface; ObS/BS, bone surface ratio; N.Ob/BS, number of osteoblasts (in cells)/unit surface; N.Oc, number of tartrate-resistant acid phosphatase-positive osteoclasts; N.Oc/BS (in cells/unit surface), cortical thickness at middiaphysis (in microns)/growth plate cartilage thickness (in microns).
a
Statistically significant difference from wild type (P < 0.05).