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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Biochemistry. 2013 Mar 8;52(11):1845–1857. doi: 10.1021/bi301568w

Figure 5.

Figure 5

The dependence of the DnaT N-terminal core domain fluorescence anisotropy upon the total domain monomer concentration (λex = 296 nm, λem = 347 nm) in buffer C (pH 7.0, 20°C) (Materials and Methods). The solid line is the nonlinear least-squares fit of the titration curve, using the dimer model described by eqs. 16 – 20, with KD = 2.1 × 107 M−1, rM = 0.055, and rT = 0.112. The dashed line is the best fit of the titration curve using monomer <-> trimer model described by eqs. 3 – 7, with KT = 2.5 × 1014 M−2, rM = 0.055, rD = 0.112.