Abstract
Certain strains of Saccharomyces cerevisiae were found to have a complex nuclear defect (designated clo-) that makes cells unable to maintain some L-B and some L-C double-stranded RNAs at 25 degrees C. The clo- strains were not defective in maintenance of L-A, M1, or M2 double-stranded RNAs. Most clo-strains lacking L and M carry small amounts of two double-stranded RNA species intermediate in size between L and M and denoted T (2.7 kilobase pairs) and W (2.25 kilobase pairs). Some strains carry both T and W, some carry neither, and some carry only W; no strains carrying only T have been found. Both T and W show 4+:0 segregation in meiosis and efficient transmission by cytoplasmic mixing (cytoduction), indicating that they are non-Mendelian genetic elements. T and W do not cross-hybridize with each other or with L-A, L-B, L-C, M1, M2, or chromosomal DNA. T and W are apparently distinct from other known non-Mendelian genetic elements (2mu DNA, [rho], [psi], 20S RNA, [URE3]). In most strains the copy number of both T and W is increased about 10-fold by the growth of cells at 37 degrees C. This heat inducibility of T and W is under control of a cytoplasmic gene. T and W double-stranded RNAs are not found in a purified L-containing virus-like particle preparation from a strain containing L-B, T, and W double-stranded RNAs. The role, if any, of T or W in the killer systems is not known.
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Selected References
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