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. 1984 Jan;4(1):188–194. doi: 10.1128/mcb.4.1.188

RNA polymerase activity in virions from Ustilago maydis.

B S Ben-Tzvi, Y Koltin, M Mevarech, A Tamarkin
PMCID: PMC368674  PMID: 6700584

Abstract

RNA polymerase activity is associated with the double-stranded RNA virions of Ustilago maydis. The reaction products of the polymerase activity are single-stranded RNA molecules. The RNA molecules synthesized are homologous to the three classes of double-stranded RNA molecules that typify the viral genome. The single-stranded RNA synthesized is released from the virions. The molecular weight of the single-stranded RNA transcripts is about half the size of the double-stranded RNA segments, and thus, it appears that in the in vitro reaction, full-length transcripts can be obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. Brennan V. E., Bobek L. A., Bruenn J. A. Yeast deRNA viral transcriptase pause products: identification of the transcript strand. Nucleic Acids Res. 1981 Oct 10;9(19):5049–5059. doi: 10.1093/nar/9.19.5049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brennan V. E., Field L., Cizdziel P., Bruenn J. A. Sequences at the 3' ends of yeast viral dsRNAs: proposed transcriptase and replicase initiation sites. Nucleic Acids Res. 1981 Aug 25;9(16):4007–4021. doi: 10.1093/nar/9.16.4007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruenn J., Bobek L., Brennan V., Held W. Yeast viral RNA polymerase is a transcriptase. Nucleic Acids Res. 1980 Jul 11;8(13):2985–2997. doi: 10.1093/nar/8.13.2985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buck K. W. Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S. Nucleic Acids Res. 1975 Oct;2(10):1889–1902. doi: 10.1093/nar/2.10.1889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carmichael G. G., McMaster G. K. The analysis of nucleic acids in gels using glyoxal and acridine orange. Methods Enzymol. 1980;65(1):380–391. doi: 10.1016/s0076-6879(80)65049-6. [DOI] [PubMed] [Google Scholar]
  7. Field L. J., Bruenn J. A., Chang T. H., Pinhasi O., Koltin Y. Two Ustilago maydis viral dsRNAs of different size code for the same product. Nucleic Acids Res. 1983 May 11;11(9):2765–2778. doi: 10.1093/nar/11.9.2765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Joklik W. K. Structure and function of the reovirus genome. Microbiol Rev. 1981 Dec;45(4):483–501. doi: 10.1128/mr.45.4.483-501.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koltin Y., Day P. R. Inheritance of killer phenotypes and double-stranded RNA in Ustilago maydis. Proc Natl Acad Sci U S A. 1976 Feb;73(2):594–598. doi: 10.1073/pnas.73.2.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koltin Y., Day P. R. Specificity of Ustilago maydis killer proteins. Appl Microbiol. 1975 Oct;30(4):694–696. doi: 10.1128/am.30.4.694-696.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koltin Y., Kandel J. S. Killer Phenomenon in USTILAGO MAYDIS: The Organization of the Viral Genome. Genetics. 1978 Feb;88(2):267–276. doi: 10.1093/genetics/88.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koltin Y., Levine R., Peery T. Assignment of functions to segments of the DsRNA genome of the Ustilago virus. Mol Gen Genet. 1980 Apr;178(1):173–178. doi: 10.1007/BF00267226. [DOI] [PubMed] [Google Scholar]
  14. Koltin Y., Mayer I., Steinlauf R. Killer phenomenon in Ustilago maydis: mapping viral functions. Mol Gen Genet. 1978 Oct 30;166(2):181–186. doi: 10.1007/BF00285920. [DOI] [PubMed] [Google Scholar]
  15. Koltin Y., Steinlauf R. The killer phenomenon in Ustilago: electron microscopy of the dsRNA encapsidated in individual virus particles. Arch Microbiol. 1980 Nov;128(1):45–52. doi: 10.1007/BF00422304. [DOI] [PubMed] [Google Scholar]
  16. Koltin Y. Virus-like particles in Ustilago maydis: mutants with partial genomes. Genetics. 1977 Jul;86(3):527–534. doi: 10.1093/genetics/86.3.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peery T., Koltin Y., Tamarkin A. Mapping the immunity function of the ustilago maydis P1 virus. Plasmid. 1982 Jan;7(1):52–58. doi: 10.1016/0147-619x(82)90026-9. [DOI] [PubMed] [Google Scholar]
  18. Silverstein S. C., Christman J. K., Acs G. The reovirus replicative cycle. Annu Rev Biochem. 1976;45:375–408. doi: 10.1146/annurev.bi.45.070176.002111. [DOI] [PubMed] [Google Scholar]
  19. Welsh D., Leibowitz M. J. Transcription of killer virion double-stranded RNA in vitro. Nucleic Acids Res. 1980 Jun 11;8(11):2365–2375. doi: 10.1093/nar/8.11.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Welsh J. D., Leibowitz M. J., Wickner R. B. Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae. Nucleic Acids Res. 1980 Jun 11;8(11):2349–2363. doi: 10.1093/nar/8.11.2349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wickner R. B., Leibowitz M. J. Dominant chromosomal mutation bypassing chromosomal genes needed for killer RNA plasmid replication in yeast. Genetics. 1977 Nov;87(3):453–469. doi: 10.1093/genetics/87.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]

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