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. 1984 Mar;4(3):559–562. doi: 10.1128/mcb.4.3.559

DNase I sensitivity of integrated simian virus 40 DNA.

G Blanck, S Chen, R Pollack
PMCID: PMC368736  PMID: 6325884

Abstract

We undertook an analysis of integrated simian virus 40 (SV40) DNA to learn whether the DNase I-sensitive region is retained in the integrated array of mouse transformants. Our results indicate that full-length integrated SV40 chromatin retains a DNase I-hypersensitive region at the same point as in nonintegrated SV40 chromatin. Thus, the lack of a DNase I-hypersensitive region is not likely to be the reason for nonpermissivity of SV40 in mouse cells. In addition, results reported here indicate that a deletion of about 200 base pairs of DNA in the region of the DNase I-hypersensitive site severely reduces the sensitivity of integrated SV40 chromatin. This result is similar to a previously reported result obtained with deletion mutants of SV40 analyzed in the lytic cycle. It is the first report of a DNA lesion affecting DNase I hypersensitivity of a mammalian chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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