Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1984 Mar;4(3):567–570. doi: 10.1128/mcb.4.3.567

Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

M M Bendig, J G Williams
PMCID: PMC368738  PMID: 6717434

Abstract

Xenopus laevis tadpole and adult beta-globin genes were injected into fertilized X. laevis eggs. Both injected genes replicated and were retained in the developing embryos with equal efficiency. Transcripts of the injected adult gene were detectable at gastrulation and reached a maximum level shortly thereafter. In contrast, transcripts of the injected tadpole gene were not detected until much later stages of development. The level of expression of both the injected genes was low compared with the level of expression of the chromosomal genes during erythropoiesis.

Full text

PDF
567

Images in this article

569Image
on p.569
569Image
on p.569

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bendig M. M. Persistence and expression of histone genes injected into Xenopus eggs in early development. Nature. 1981 Jul 2;292(5818):65–67. doi: 10.1038/292065a0. [DOI] [PubMed] [Google Scholar]
  2. Bendig M. M., Williams J. G. Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6197–6201. doi: 10.1073/pnas.80.20.6197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  4. Devine J. M., Tsang A. S., Williams J. G. Differential expression of the members of the discoidin I multigene family during growth and development of Dictyostelium discoideum. Cell. 1982 Apr;28(4):793–800. doi: 10.1016/0092-8674(82)90058-7. [DOI] [PubMed] [Google Scholar]
  5. Etkin L. D., Roberts M. Transmission of integrated sea urchin histone genes by nuclear transplantation in Xenopus laevis. Science. 1983 Jul 1;221(4605):67–69. doi: 10.1126/science.6857265. [DOI] [PubMed] [Google Scholar]
  6. Hentschel C. C., Kay R. M., Williams J. G. Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA. Dev Biol. 1979 Oct;72(2):350–363. doi: 10.1016/0012-1606(79)90124-6. [DOI] [PubMed] [Google Scholar]
  7. Hosbach H. A., Wyler T., Weber R. The Xenopus laevis globin gene family: chromosomal arrangement and gene structure. Cell. 1983 Jan;32(1):45–53. doi: 10.1016/0092-8674(83)90495-6. [DOI] [PubMed] [Google Scholar]
  8. Patient R. K., Elkington J. A., Kay R. M., Williams J. G. Internal organization of the major adult alpha- and beta-globin genes of X. laevis. Cell. 1980 Sep;21(2):565–573. doi: 10.1016/0092-8674(80)90494-8. [DOI] [PubMed] [Google Scholar]
  9. Rusconi S., Schaffner W. Transformation of frog embryos with a rabbit beta-globin gene. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5051–5055. doi: 10.1073/pnas.78.8.5051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sharp P. A., Berk A. J., Berget S. M. Transcription maps of adenovirus. Methods Enzymol. 1980;65(1):750–768. doi: 10.1016/s0076-6879(80)65071-x. [DOI] [PubMed] [Google Scholar]
  11. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES