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Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1984 Apr;4(4):591–598. doi: 10.1128/mcb.4.4.591

Repetitive Dictyostelium heat-shock promotor functions in Saccharomyces cerevisiae.

J Cappello, C Zuker, H F Lodish
PMCID: PMC368762  PMID: 6371492

Abstract

The Dictyostelium genome contains 40 copies of a 4.7-kilobase repetitive and apparently transposable DNA sequence (DIRS-1) and about 250 smaller elements that appear to be deletions or rearrangements of DIRS-1. Transcripts of these sequences are induced during differentiation and also by heat shock treatment of growing cells. We showed that one such cloned element, pB41.6 (2.5 kilobases) contains a nucleotide sequence identical to the Drosophila consensus heat shock promotor. To test whether this sequence might indeed control the expression of DIRS-1-related RNAs, we have cloned this genomic segment into yeast cells. In yeast cells, 41.6 directs synthesis of a 1.7-kilobase RNA that is induced at least 10-fold by heat shock. Transcription initiates at about 124 bases 3' of the putative promotor sequence and terminates within the 41.6 insert. A 381-base-pair subclone that contains the putative promotor sequence is sufficient to induce the heat shock response of 41.6 in yeast cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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