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. 1984 Apr;4(4):666–670. doi: 10.1128/mcb.4.4.666

Reacquisition of a functional early region by a mouse transformant containing only defective simian virus 40 DNA.

S Chen, G Blanck, R Pollack
PMCID: PMC368778  PMID: 6325888

Abstract

Viral DNA in simian virus 40-transformed mouse cells is capable of rearranging with passage. In this report, we show that such rearrangement can include an alteration in viral protein expression. SVT2, a simian virus 40-transformed mouse BALB/c 3T3 cell line, synthesizes only a super T antigen of molecular weight 100,000 without synthesizing the lytic-size large T or small t antigens with molecular weights of 94,000 and 17,000, respectively. Analyses of the integrated viral DNA revealed an early region of 4.4 kilobases instead of the lytic-size 2.7 kilobases. However, upon subcloning in either plastic or agarose or after being in culture for several passages, the appearance of lytic-size large T and small t antigens was detected. Concurrently, an early region of 2.7 kilobases, in addition to one of 4.4 kilobases, was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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