Skip to main content
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1984 May;4(5):985–988. doi: 10.1128/mcb.4.5.985

5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.

J H Proffitt, J R Davie, D Swinton, S Hattman
PMCID: PMC368855  PMID: 6374428

Abstract

We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.

Full text

PDF
985

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. L., Burdon R. H. DNA methylation in eukaryotes. CRC Crit Rev Biochem. 1982;13(4):349–384. doi: 10.3109/10409238209108714. [DOI] [PubMed] [Google Scholar]
  2. Adams R. L., McKay E. L., Craig L. M., Burdon R. H. Methylation of mosquito DNA. Biochim Biophys Acta. 1979 Jun 20;563(1):72–81. doi: 10.1016/0005-2787(79)90008-x. [DOI] [PubMed] [Google Scholar]
  3. Cummings D. J., Tait A., Goddard J. M. Methylated bases in DNA from Paramecium aurelia. Biochim Biophys Acta. 1974 Nov 20;374(1):1–11. doi: 10.1016/0005-2787(74)90194-4. [DOI] [PubMed] [Google Scholar]
  4. Doerfler W. DNA methylation--a regulatory signal in eukaryotic gene expression. J Gen Virol. 1981 Nov;57(Pt 1):1–20. doi: 10.1099/0022-1317-57-1-1. [DOI] [PubMed] [Google Scholar]
  5. Dybvig K., Swinton D., Maniloff J., Hattman S. Cytosine methylation of the sequence GATC in a mycoplasma. J Bacteriol. 1982 Sep;151(3):1420–1424. doi: 10.1128/jb.151.3.1420-1424.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehrlich M., Wang R. Y. 5-Methylcytosine in eukaryotic DNA. Science. 1981 Jun 19;212(4501):1350–1357. doi: 10.1126/science.6262918. [DOI] [PubMed] [Google Scholar]
  7. Gama-Sosa M. A., Wang R. Y., Kuo K. C., Gehrke C. W., Ehrlich M. The 5-methylcytosine content of highly repeated sequences in human DNA. Nucleic Acids Res. 1983 May 25;11(10):3087–3095. doi: 10.1093/nar/11.10.3087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorovsky M. A., Hattman S., Pleger G. L. ( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis. J Cell Biol. 1973 Mar;56(3):697–701. doi: 10.1083/jcb.56.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartley J. L., Donelson J. E. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860–865. doi: 10.1038/286860a0. [DOI] [PubMed] [Google Scholar]
  10. Hattman S., Kenny C., Berger L., Pratt K. Comparative study of DNA methylation in three unicellular eucaryotes. J Bacteriol. 1978 Sep;135(3):1156–1157. doi: 10.1128/jb.135.3.1156-1157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKnight G. L., Cardillo T. S., Sherman F. An extensive deletion causing overproduction of yeast iso-2-cytochrome c. Cell. 1981 Aug;25(2):409–419. doi: 10.1016/0092-8674(81)90059-3. [DOI] [PubMed] [Google Scholar]
  12. Pratt K., Hattman S. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila. Mol Cell Biol. 1981 Jul;1(7):600–608. doi: 10.1128/mcb.1.7.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rae P. M., Steele R. E. Modified bases in the DNAs of unicellular eukaryotes: an examination of distributions and possible roles, with emphasis on hydroxymethyluracil in dinoflagellates. Biosystems. 1978 Apr;10(1-2):37–53. doi: 10.1016/0303-2647(78)90027-8. [DOI] [PubMed] [Google Scholar]
  14. Razin A., Riggs A. D. DNA methylation and gene function. Science. 1980 Nov 7;210(4470):604–610. doi: 10.1126/science.6254144. [DOI] [PubMed] [Google Scholar]
  15. Singhal R. P. Ion-exlusion chromatography: analysis and isolation of nucleic acid components, and influence of separation parameters. Arch Biochem Biophys. 1972 Oct;152(2):800–810. doi: 10.1016/0003-9861(72)90276-7. [DOI] [PubMed] [Google Scholar]
  16. Urieli-Shoval S., Gruenbaum Y., Sedat J., Razin A. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett. 1982 Sep 6;146(1):148–152. doi: 10.1016/0014-5793(82)80723-0. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES